Corrêa Luciano Jacob, Badino Alberto Colli, Cruz Antonio José Gonçalves
PPGEQ/UFSCar, Chemical Engineering Graduate Program, Federal University of São Carlos, São Paulo, Brazil.
Department of Chemical Engineering, Federal University of São Carlos, São Carlos, Brazil.
Bioprocess Biosyst Eng. 2016 May;39(5):825-33. doi: 10.1007/s00449-016-1562-4. Epub 2016 Feb 22.
The minimization of costs in the distillation step of lignocellulosic ethanol production requires the use of a high solids loading during the enzymatic hydrolysis to obtain a more concentrated glucose liquor. However, this increase in biomass can lead to problems including increased mass and heat transfer resistance, decreased cellulose conversion, and increased apparent viscosity with the associated increase in power consumption. The use of fed-batch operation offers a promising way to circumvent these problems. In this study, one batch and four fed-batch strategies for solids and/or enzyme feeding during the enzymatic hydrolysis of sugarcane bagasse were evaluated. Determinations of glucose concentration, power consumption, and apparent viscosity were made throughout the experiments, and the different strategies were compared in terms of energy efficiency (mass of glucose produced according to the energy consumed). The best energy efficiency was obtained for the strategy in which substrate and enzyme were added simultaneously (0.35 kg(glucose) kWh⁻¹). This value was 52% higher than obtained in batch operation.
在木质纤维素乙醇生产的蒸馏步骤中，成本最小化要求在酶水解过程中使用高固含量，以获得更浓缩的葡萄糖溶液。然而，生物质的这种增加可能会导致一些问题，包括传质和传热阻力增加、纤维素转化率降低以及表观粘度增加，同时功耗也会增加。采用补料分批操作提供了一种有望规避这些问题的方法。在本研究中，评估了甘蔗渣酶水解过程中一种固体和/或酶进料的分批策略以及四种补料分批策略。在整个实验过程中测定了葡萄糖浓度、功耗和表观粘度，并根据能源效率（根据消耗的能量产生的葡萄糖质量）对不同策略进行了比较。底物和酶同时添加的策略获得了最佳能源效率（0.35 kg(葡萄糖) kWh⁻¹）。该值比分批操作获得的值高52%。
