Yu Deliang, Ji Chenglong, Zhao Jianmin, Wu Huifeng
Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.
Chemosphere. 2016 May;150:194-201. doi: 10.1016/j.chemosphere.2016.01.113. Epub 2016 Feb 20.
Inorganic arsenic (As) is a known pollutant including two chemical forms (arsenite (As III) and arsenate (As V)), in marine and coastal environment. Marine mussel Mytilus galloprovincialis is an important environmental monitoring species around the world. In this study, we focused on valence-specific responses of As in juvenile mussel M. galloprovincialis using a combined proteomic and metabolomic approach. Metabolic responses indicated that As (III) mainly caused disturbance in osmotic regulation in juvenile mussels. As (V) caused disturbances in both osmotic regulation and energy metabolism marked by different metabolic responses, including betaine, taurine, glucose and glycogen. Proteomic responses exhibited that As (III) had a significant negative effect on cytoskeleton and cell structure (actin and collagen alpha-6(VI) chain). As (V) affected some key enzymes involved in energy metabolism (cytosolic malate dehydrogenase, cMDH) and cell development (ornithine aminotransferase and astacin). Overall, all these results confirmed the valence-specific responses in juvenile mussels to As exposures. These findings demonstrate that a combined metabolomic and proteomic approach could provide an important insight into the toxicological effects of environmental pollutants in organisms.
无机砷（As）是一种已知的污染物，在海洋和沿海环境中有两种化学形态（亚砷酸盐（As III）和砷酸盐（As V））。海洋贻贝加利福尼亚贻贝是全球重要的环境监测物种。在本研究中，我们采用蛋白质组学和代谢组学相结合的方法，重点研究了幼年加利福尼亚贻贝对不同价态砷的特异性反应。代谢反应表明，As（III）主要导致幼年贻贝的渗透调节紊乱。As（V）通过不同的代谢反应，包括甜菜碱、牛磺酸、葡萄糖和糖原，导致渗透调节和能量代谢紊乱。蛋白质组学反应显示，As（III）对细胞骨架和细胞结构（肌动蛋白和胶原蛋白α-6(VI)链）有显著负面影响。As（V）影响参与能量代谢（胞质苹果酸脱氢酶，cMDH）和细胞发育（鸟氨酸转氨酶和虾红素）的一些关键酶。总体而言，所有这些结果证实了幼年贻贝对砷暴露的价态特异性反应。这些发现表明，代谢组学和蛋白质组学相结合的方法可以为深入了解环境污染物对生物体的毒理学效应提供重要线索。
