Kawasaki Takeru, Narulita Erlia, Matsunami Minaho, Ishikawa Hiroki, Shimizu Mio, Fujie Makoto, Bhunchoth Anjana, Phironrit Namthip, Chatchawankanphanich Orawan, Yamada Takashi
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan.
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima 739-8530, Japan; Study Program of Biology Education, University of Jember, Jember 68121, Indonesia.
Virology. 2016 May;492:73-81. doi: 10.1016/j.virol.2016.02.011. Epub 2016 Feb 19.
The genome organization, gene structure, and host range of five podoviruses that infect Ralstonia solanacearum, the causative agent of bacterial wilt disease were characterized. The phages fell into two distinctive groups based on the genome position of the RNA polymerase gene (i.e., T7-type and ϕKMV-type). One-step growth experiments revealed that ϕRSB2 (a T7-like phage) lysed host cells more efficiently with a shorter infection cycle (ca. 60 min corresponding to half the doubling time of the host) than ϕKMV-like phages such as ϕRSB1 (with an infection cycle of ca. 180 min). Co-infection experiments with ϕRSB1 and ϕRSB2 showed that ϕRSB2 always predominated in the phage progeny independent of host strains. Most phages had wide host-ranges and the phage particles usually did not attach to the resistant strains; when occasionally some did, the phage genome was injected into the resistant strain's cytoplasm, as revealed by fluorescence microscopy with SYBR Gold-labeled phage particles.
对五种感染青枯雷尔氏菌（细菌性枯萎病的病原体）的短尾病毒的基因组组织、基因结构和宿主范围进行了表征。根据RNA聚合酶基因的基因组位置，这些噬菌体分为两个不同的组（即T7型和ϕKMV型）。一步生长实验表明，ϕRSB2（一种T7样噬菌体）比ϕKMV样噬菌体（如ϕRSB1，感染周期约为180分钟）更有效地裂解宿主细胞，感染周期更短（约60分钟，相当于宿主倍增时间的一半）。用ϕRSB1和ϕRSB2进行的共感染实验表明，无论宿主菌株如何，ϕRSB2在噬菌体后代中总是占主导地位。大多数噬菌体具有广泛的宿主范围，噬菌体颗粒通常不附着于抗性菌株；如用SYBR Gold标记的噬菌体颗粒进行荧光显微镜观察所示，偶尔有些噬菌体附着时，噬菌体基因组会注入抗性菌株的细胞质中。
