Azevedo Andreia S, Almeida Carina, Pereira Bruno, Melo Luís F, Azevedo Nuno F
a Laboratory for Process Engineering, Environment, and Energy and Biotechnology Engineering (LEPABE), Faculty of Engineering, Department of Chemical Engineering , University of Porto , Porto , Portugal.
b Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy , University of Southern Denmark , Odense M , Denmark.
Biofouling. 2016;32(3):227-41. doi: 10.1080/08927014.2015.1124096.
Recently it was demonstrated that for urinary tract infections species with a lower or unproven pathogenic potential, such as Delftia tsuruhatensis and Achromobacter xylosoxidans, might interact with conventional pathogenic agents such as Escherichia coli. Here, single- and dual-species biofilms of these microorganisms were characterized in terms of microbial composition over time, the average fitness of E. coli, the spatial organization and the biofilm antimicrobial profile. The results revealed a positive impact of these species on the fitness of E. coli and a greater tolerance to the antibiotic agents. In dual-species biofilms exposed to antibiotics, E. coli was able to dominate the microbial consortia in spite of being the most sensitive strain. This is the first study demonstrating the protective effect of less common species over E. coli under adverse conditions imposed by the use of antibiotic agents.
最近有研究表明，对于尿路感染，一些致病潜力较低或未经证实的菌种，如鹤见德尔夫特菌和木糖氧化无色杆菌，可能会与传统病原体如大肠杆菌相互作用。在此，对这些微生物的单菌种和双菌种生物膜在微生物组成随时间的变化、大肠杆菌的平均适应性、空间组织以及生物膜抗菌谱方面进行了表征。结果显示这些菌种对大肠杆菌的适应性有积极影响，并且对抗生素具有更高的耐受性。在暴露于抗生素的双菌种生物膜中，尽管大肠杆菌是最敏感的菌株，但它仍能够在微生物群落中占据主导地位。这是第一项证明在使用抗生素所施加的不利条件下，较不常见的菌种对大肠杆菌具有保护作用的研究。
