Adesina T D, Nwinyi O C, Olugbuyiro J A O
Pak J Biol Sci. 2015 Feb;18(2):67-73. doi: 10.3923/pjbs.2015.67.73.
In this study, we investigated the feasibility of using Psidium guajava, Mangifera indica and Ocimum gratissimum leaf extracts in preventing Escherichia coli biofilm formation. The plants extractions were done with methanol under cold extraction. The various concentrations 5.0, 10.0 and 20.0 mg mL(-1) were used to coat 63 catheters under mild heat from water bath. Biofilm formation on the catheter was induced using cultures of E. coli. Biofilm formation was evaluated using aerobic plate count and turbidity at 600 nm. From the obtained results, Psidium guajava, Mangifera indica and Ocimum gratissimum delayed the onset of biofilm formation for a week. Ocimum gratissimum coated catheter had the highest inhibitory effect at 5.0, 10.0 and 20.0 mg mL(-1) with bacterial count ranging from 2.2 x 10(5)-7.0 x 10(4) and 5.7 x 10(5)-3.7 x10(5) for 120 and 128 h, respectively. The Psidium guajava coated catheter had the lowest inhibitory effect at 5.0, 10.0 and 20.0 mg mL(-1), with bacterial count ranging between 4.3 x 10(5)-1.9 x 10(3) and 7.7 x 10(5)-3.8 x 10(5) for 120 and 128 h, respectively. Despite the antimicrobial activities, the differences in the activity of these plant extracts were statistically not significant (p < 0.05).
在本研究中，我们调查了使用番石榴、芒果和罗勒叶提取物预防大肠杆菌生物膜形成的可行性。植物提取物采用甲醇冷提取法制备。使用5.0、10.0和20.0 mg mL(-1)的不同浓度在水浴微热条件下包被63根导管。使用大肠杆菌培养物诱导导管上的生物膜形成。使用需氧平板计数法和600 nm处的吸光度评估生物膜形成情况。从所得结果来看，番石榴、芒果和罗勒延迟了生物膜形成一周。罗勒包被的导管在5.0、10.0和20.0 mg mL(-1)浓度下具有最高的抑制作用，在120小时和128小时时细菌计数分别为2.2×10(5)-7.0×10(4)和5.7×10(5)-3.7×10(5)。番石榴包被的导管在5.0、10.0和20.0 mg mL(-1)浓度下具有最低的抑制作用，在120小时和128小时时细菌计数分别为4.3×10(5)-1.9×10(3)和7.7×10(5)-3.8×10(5)。尽管这些植物提取物具有抗菌活性，但这些提取物活性的差异在统计学上并不显著（p<0.05）。
