Okadera Kota, Abe Masako, Ito Naoto, Mitake Hiromichi, Okada Kazuma, Nakagawa Kento, Une Yumi, Tsunemitsu Hiroshi, Sugiyama Makoto
The United Graduate School of Veterinary Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
Laboratory of Zoonotic Diseases, Faculty of Applied Biological Sciences,Gifu University, 1-1 Yanagido, Gifu 501-1193,Japan.
J Gen Virol. 2016 May;97(5):1158-1167. doi: 10.1099/jgv.0.000433. Epub 2016 Feb 19.
To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders (Petaurus breviceps) was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated. All of the genes of the strain were classified as new genotypes (G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12). The enterotoxin domain in NSP4, which is important for the induction of diarrhoea, was conserved between SG385-tc and previously reported mammalian strains, suggesting the potential of sugar glider RVA to cause diarrhoea in mammalian species. In fact, seven out of nine suckling mice inoculated orally with 3.9 × 104 f.f.u. of strain SG385-tc had diarrhoea and the 50 % diarrhoea-inducing dose (DD50) of strain SG385-tc in suckling mice was 1.2 × 104 f.f.u. Our findings suggest that sugar glider RVA is infective to and possibly pathogenic in other mammalian species.
为评估A组轮状病毒（RVA）从外来宠物跨物种传播至其他哺乳动物物种的风险，研究了蜜袋鼯（Petaurus breviceps）中RVA的流行情况。通过逆转录（RT）-半巢式PCR在44只蜜袋鼯中的10只检测到RVA。这些病毒被分类为G27P[3]和G27P[36]基因型，其中G27和P[36]是轮状病毒分类工作组指定的新基因型。为详细表征蜜袋鼯RVA，分离出一株病毒，即RVA/蜜袋鼯-tc/日本/SG385/2012/G27P[36]（SG385-tc）。该毒株的所有基因均被分类为新基因型（G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12）。NSP4中的肠毒素结构域对腹泻的诱导很重要，在SG385-tc和先前报道的哺乳动物毒株之间保守，这表明蜜袋鼯RVA有可能在哺乳动物物种中引起腹泻。事实上，口服接种3.9×104个感染性病毒单位（f.f.u.）的SG385-tc毒株的9只乳鼠中有7只出现腹泻，SG385-tc毒株在乳鼠中的50%腹泻诱导剂量（DD50）为1.2×104 f.f.u.。我们的研究结果表明，蜜袋鼯RVA可感染其他哺乳动物物种并可能具有致病性。
