Roger Tchikoua, Ngouné Léopold Tatsadjieu, Carl Moses Funtong Mbofung
National School of Agro-Industrial Sciences, Ngaoundere, Cameroon.
University Institute of Technology, University of Ngaoundere, Ngaoundere, Cameroon.
Int J Food Sci. 2015;2015:502910. doi: 10.1155/2015/502910. Epub 2015 May 19.
The aim of this study is to reduce antinutritional factors and to improve the nutritional properties of Kutukutu during fermentation with Lactic Acid Bacteria (LAB). For that, Kutukutu (700 g) was prepared in the laboratory and inoculated with pure cultures of LAB (10(9) CFU/mL). Then, preparation was incubated for 120 h. Every 24 h, Kutukutu were collected, dried at 45°C for 24 h, and analyzed. The results showed that Lactobacillus brevis G25 increased reducing sugars content to 80.7% in Kutukutu after 96 h of fermentation. Lactobacillus fermentum N33 reduced the starch content to 73.2%, while Lactobacillus brevis G11, L. brevis G25, and Lactobacillus cellobiosus M41 rather increased the protein content to 18.9%. The bioavailability of Mg and Fe increased, respectively, to 50.5% and 70.6% in the Kutukutu fermented with L. brevis G25. L. plantarum A6 reduced the tannin content to 98.8% and L. buchneri M11 reduced the phytate content to 95.5%. The principal component analysis (PCA) shows that, for a best reduction of antinutrients factors and improvement of protein content and minerals, Kutukutu must be fermented by L. brevis G25 and L. fermentum N33, respectively. These starter cultures could be used to ameliorate nutritional proprieties of Kutukutu during the fermentation.
本研究的目的是在乳酸菌(LAB)发酵过程中降低抗营养因子,并改善库图库图(Kutukutu)的营养特性。为此,在实验室制备了700克库图库图,并接种了乳酸菌纯培养物(10⁹CFU/mL)。然后,将制剂孵育120小时。每隔24小时收集库图库图,在45°C下干燥24小时并进行分析。结果表明,短乳杆菌G25在发酵96小时后使库图库图中的还原糖含量增加到80.7%。发酵乳杆菌N33将淀粉含量降低到73.2%,而短乳杆菌G11、短乳杆菌G25和纤维二糖乳杆菌M41则将蛋白质含量提高到18.9%。在用短乳杆菌G25发酵的库图库图中,镁和铁的生物利用率分别提高到50.5%和70.6%。植物乳杆菌A6将单宁含量降低到98.8%,布氏乳杆菌M11将植酸含量降低到95.5%。主成分分析(PCA)表明,为了最佳地降低抗营养因子并提高蛋白质含量和矿物质含量,库图库图必须分别用短乳杆菌G25和发酵乳杆菌N33进行发酵。这些发酵剂可用于改善库图库图发酵过程中的营养特性。