Vaintraub I A, Lapteva N A
Laboratory of Protein Chemistry, V.I. Lenin State University of Kishiney, USSR.
Anal Biochem. 1988 Nov 15;175(1):227-30. doi: 10.1016/0003-2697(88)90382-x.
The addition of orthophosphate (up to 20 micrograms/ml of phosphorus) and chlorogenic acid (up to 50 micrograms/ml) does not impair the colorimetric assay of phytate based on the decoloration of Fe3+-sulfosalicylate complex (M. Latta, and M. Eskin (1980) J. Agric. Food Chem. 28, 1313-1315). Phytate was determined in 14 samples of seed meal and protein isolates containing inorganic phosphate and chlorogenic acid. There was no difference between the results of the analysis using crude extracts and those using purified extracts. It is therefore possible to avoid the preliminary purification of extracts as in the original method, thereby simplifying and accelerating the phytate assay to a considerable extent.
添加正磷酸盐（磷含量高达20微克/毫升）和绿原酸（含量高达50微克/毫升）不会影响基于Fe3 + -磺基水杨酸络合物褪色的植酸盐比色测定法（M. Latta和M. Eskin（1980年），《农业与食品化学杂志》28卷，1313 - 1315页）。对14个含有无机磷酸盐和绿原酸的籽粕和蛋白质分离物样品中的植酸盐进行了测定。使用粗提物的分析结果与使用纯化提取物的分析结果之间没有差异。因此，有可能像原始方法那样避免提取物的初步纯化，从而在很大程度上简化并加速植酸盐测定。
