Mercier Anne, Bertaux Joanne, Lesobre Jérôme, Gravouil Kevin, Verdon Julien, Imbert Christine, Valette Eric, Héchard Yann
a Equipe Microbiologie de l'Eau, Ecologie et Biologie des Interactions, Centre National de la Recherche Scientifique UMR 7267 , Université de Poitiers , Poitiers , France.
b Equipe Ecologie Evolution Symbiose, Ecologie et Biologie des Interactions, Centre National de la Recherche Scientifique UMR 7267 , Université de Poitiers , Poitiers , France.
Biofouling. 2016;32(3):287-99. doi: 10.1080/08927014.2015.1137896.
Electromagnetic field (EMF) treatment has proven to be effective against mineral scaling in water systems. Therefore, it should be assessed for the treatment of other deposits such as biofilms. In this study, a commercial device producing low-frequency EMF (1-10 kHz) was applied to a reactor fed with natural water for 45 days. The treatment promoted the concentration of microorganisms in suspension and limited the amount of sessile microorganisms in the biofilm, as determined by the measurement of total DNA, qPCR and microscopy. The structure of the bacterial community was assessed by t-RFLP and pyrosequencing analysis. The results showed that EMF treatment affected both planktonic and sessile community composition. EMFs were responsible for a shift in classes of Proteobacteria during development of the biofilm. It may be speculated that the EMF treatment affected particle solubility and/or microorganism hydration. This study indicated that EMFs modulated biofilm formation in natural water.
电磁场（EMF）处理已被证明对水系统中的矿物结垢有效。因此，应评估其对生物膜等其他沉积物的处理效果。在本研究中，将一台产生低频电磁场（1 - 10 kHz）的商用设备应用于一个以天然水为进水的反应器，持续45天。通过测量总DNA、定量聚合酶链反应（qPCR）和显微镜观察确定，该处理促进了悬浮微生物的浓度，并限制了生物膜中固着微生物的数量。通过末端限制性片段长度多态性分析（t - RFLP）和焦磷酸测序分析评估细菌群落结构。结果表明，电磁场处理影响了浮游和固着群落的组成。在生物膜形成过程中，电磁场导致了变形菌门各纲的转变。可以推测，电磁场处理影响了颗粒的溶解度和/或微生物的水合作用。本研究表明，电磁场调节了天然水中生物膜的形成。
