Li Senwu, Yang Kaiguang, Deng Nan, Min Yi, Liu Lukuan, Zhang Lihua, Zhang Yukui
National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences , Dalian 116023, China.
University of Chinese Academy of Sciences , Beijing 100049, China.
ACS Appl Mater Interfaces. 2016 Mar 9;8(9):5747-51. doi: 10.1021/acsami.5b11415. Epub 2016 Feb 26.
Among various artificial antibodies, epitope imprinted polymer has been paid increasingly attention. To modulate the "adsorption and release" behavior by environment stimuli, N-isopropylacrylamide, was adopted to fabricate the thermoresponsive epitope imprinted sites. The prepared imprinted materials could adsorb 46.6 mg/g of target protein with the imprinting factor of 4.0. The template utilization efficiency could reach as high as 8.21%. More importantly, in the real sample, the materials could controllably capture the target protein from the human plasma at 45 °C and release it at 4 °C, which demonstrated the "on-demand" application potentials of such materials in the biomolecule recognition field.
在各种人工抗体中，表位印迹聚合物越来越受到关注。为了通过环境刺激调节“吸附和释放”行为，采用N-异丙基丙烯酰胺制备了热响应性表位印迹位点。制备的印迹材料能够吸附46.6 mg/g的目标蛋白，印迹因子为4.0。模板利用效率高达8.21%。更重要的是，在实际样品中，该材料能够在45℃下从人血浆中可控地捕获目标蛋白，并在4℃下释放，这证明了此类材料在生物分子识别领域的“按需”应用潜力。
