基于表位印迹复合纳米颗粒的细胞疗法，从体外模型中去除α-突触核蛋白。

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model.

机构信息

Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.

Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.

出版信息

Cells. 2022 Aug 19;11(16):2584. doi: 10.3390/cells11162584.

DOI:10.3390/cells11162584

PMID:36010659

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9406856/

Abstract

Several degenerative disorders of the central nervous system, including Parkinson's disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer-Emmett-Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.

摘要

几种中枢神经系统退行性疾病，包括帕金森病（PD），与蛋白质的病理性聚集有关。因此，针对有毒疾病蛋白（如α-突触核蛋白（SNCA））的抗体被开发为潜在的治疗方法。在这项工作中，使用 SNCA 中的一个肽段（YVGSKTKEGVVHGVA）作为抗原来构建磁性分子印迹复合纳米颗粒（MMIPs）。这些复合纳米颗粒通过动态光散射（DLS）、高效液相色谱（HPLC）、等温热力学滴定（ITC）、BET 分析和超导量子干涉器件（SQUID）分析进行了表征。最后，测量了用 MMIPs 处理的脑内皮细胞的活力，并从体外模型系统中的 CRISPR/dCas9a 激活的 HEK293T 细胞中提取了 SNCA，以展示 MMIPs 的治疗应用。