• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于表位印迹复合纳米颗粒的细胞疗法，从体外模型中去除α-突触核蛋白。

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model.

机构信息

Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan.

Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan.

出版信息

Cells. 2022 Aug 19;11(16):2584. doi: 10.3390/cells11162584.

DOI:10.3390/cells11162584
PMID:36010659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9406856/
Abstract

Several degenerative disorders of the central nervous system, including Parkinson's disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer-Emmett-Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.

摘要

几种中枢神经系统退行性疾病，包括帕金森病（PD），与蛋白质的病理性聚集有关。因此，针对有毒疾病蛋白（如α-突触核蛋白（SNCA））的抗体被开发为潜在的治疗方法。在这项工作中，使用 SNCA 中的一个肽段（YVGSKTKEGVVHGVA）作为抗原来构建磁性分子印迹复合纳米颗粒（MMIPs）。这些复合纳米颗粒通过动态光散射（DLS）、高效液相色谱（HPLC）、等温热力学滴定（ITC）、BET 分析和超导量子干涉器件（SQUID）分析进行了表征。最后，测量了用 MMIPs 处理的脑内皮细胞的活力，并从体外模型系统中的 CRISPR/dCas9a 激活的 HEK293T 细胞中提取了 SNCA，以展示 MMIPs 的治疗应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/71ef1c681167/cells-11-02584-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/4fbf154588e7/cells-11-02584-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/5f0e8d20ad45/cells-11-02584-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/ecb709118d21/cells-11-02584-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/dee1eacd2018/cells-11-02584-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/22174200255e/cells-11-02584-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/5b65d589e06a/cells-11-02584-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/71ef1c681167/cells-11-02584-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/4fbf154588e7/cells-11-02584-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/5f0e8d20ad45/cells-11-02584-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/ecb709118d21/cells-11-02584-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/dee1eacd2018/cells-11-02584-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/22174200255e/cells-11-02584-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/5b65d589e06a/cells-11-02584-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7841/9406856/71ef1c681167/cells-11-02584-g006.jpg

相似文献

1
Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model.基于表位印迹复合纳米颗粒的细胞疗法，从体外模型中去除α-突触核蛋白。
Cells. 2022 Aug 19;11(16):2584. doi: 10.3390/cells11162584.
2
Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.磁性肽印迹壳聚糖复合纳米颗粒对抗原的识别用于从转染细胞中提取 CRISPR/dCas9a 蛋白。
Nanotechnology. 2021 Apr 30;32(18):18LT02. doi: 10.1088/1361-6528/abde00.
3
Hydrolysis of magnetic amylase-imprinted poly(ethylene-co-vinyl alcohol) composite nanoparticles.磁性淀粉酶印迹聚（乙烯-co-乙烯醇）复合纳米粒子的水解。
ACS Appl Mater Interfaces. 2012 Feb;4(2):916-21. doi: 10.1021/am201576y. Epub 2012 Feb 9.
4
Immunotherapy of Hepatocellular Carcinoma with Magnetic PD-1 Peptide-Imprinted Polymer Nanocomposite and Natural Killer Cells.磁性 PD-1 肽印迹聚合物纳米复合材料和自然杀伤细胞治疗肝细胞癌的免疫疗法。
Biomolecules. 2019 Oct 25;9(11):651. doi: 10.3390/biom9110651.
5
Nano-sized magnetic core-shell and bulk molecularly imprinted polymers for selective extraction of amiodarone from human plasma.纳米级磁性核壳和整体分子印迹聚合物用于选择性从人血浆中提取胺碘酮。
Anal Chim Acta. 2022 Mar 15;1198:339548. doi: 10.1016/j.aca.2022.339548. Epub 2022 Jan 28.
6
Development of magnetic molecularly imprinted polymers based on carbon nanotubes - application for trace analysis of pyrethroids in fruit matrices.基于碳纳米管的磁性分子印迹聚合物的研制——在水果基质中拟除虫菊酯痕量分析中的应用。
J Chromatogr A. 2014 Feb 14;1329:1-9. doi: 10.1016/j.chroma.2013.12.079. Epub 2014 Jan 2.
7
Magnetic molecularly imprinted polymers for extraction of S-phenylmercapturic acid from urine samples followed by high-performance liquid chromatography.磁性分子印迹聚合物用于从尿液样本中提取 S-苯巯基尿酸，然后进行高效液相色谱分析。
J Mol Recognit. 2021 Nov;34(11):e2930. doi: 10.1002/jmr.2930. Epub 2021 Aug 25.
8
Magnetic molecularly imprinted polymers prepared by reversible addition fragmentation chain transfer polymerization for dispersive solid phase extraction of polycyclic aromatic hydrocarbons in water.通过可逆加成-断裂链转移聚合制备的磁性分子印迹聚合物用于水中多环芳烃的分散固相萃取。
J Chromatogr A. 2020 Jan 11;1610:460534. doi: 10.1016/j.chroma.2019.460534. Epub 2019 Sep 7.
9
Synthesis of Multifunctional Nanoparticles for the Combination of Photodynamic Therapy and Immunotherapy.用于光动力疗法与免疫疗法联合的多功能纳米颗粒的合成
Pharmaceuticals (Basel). 2021 May 26;14(6):508. doi: 10.3390/ph14060508.
10
Epitope imprinting of alpha-synuclein for sensing in Parkinson's brain organoid culture medium.用于在帕金森病脑类器官培养基中进行检测的α-突触核蛋白的表位印记
Biosens Bioelectron. 2021 Mar 1;175:112852. doi: 10.1016/j.bios.2020.112852. Epub 2020 Nov 27.

引用本文的文献

1
Recent Advances in Molecularly Imprinted Polymers and Their Disease-Related Applications.分子印迹聚合物及其疾病相关应用的最新进展
Polymers (Basel). 2023 Oct 24;15(21):4199. doi: 10.3390/polym15214199.
2
The Use of Sensors in Blood-Brain Barrier-on-a-Chip Devices: Current Practice and Future Directions.传感器在脑血屏障芯片设备中的应用：当前实践和未来方向。
Biosensors (Basel). 2023 Mar 8;13(3):357. doi: 10.3390/bios13030357.
3
Molecularly Imprinted Nanomaterials with Stimuli Responsiveness for Applications in Biomedicine.分子印迹纳米材料的刺激响应及其在生物医学中的应用。

本文引用的文献

1
Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein.基于分子印迹聚合物的扩展栅场效应晶体管（EG-FET）化学传感器，用于选择性测定基质金属蛋白酶-1（MMP-1）蛋白。
Biosens Bioelectron. 2022 Jul 15;208:114203. doi: 10.1016/j.bios.2022.114203. Epub 2022 Mar 22.
2
Doping of MXenes enhances the electrochemical response of peptide-imprinted conductive polymers for the recognition of C-Reactive protein.MXenes 的掺杂增强了肽印迹导电聚合物的电化学响应，用于 C-反应蛋白的识别。
Biosens Bioelectron. 2022 Mar 15;200:113930. doi: 10.1016/j.bios.2021.113930. Epub 2021 Dec 28.
3
Molecules. 2023 Jan 17;28(3):918. doi: 10.3390/molecules28030918.
Synthesis of Multifunctional Nanoparticles for the Combination of Photodynamic Therapy and Immunotherapy.
用于光动力疗法与免疫疗法联合的多功能纳米颗粒的合成
Pharmaceuticals (Basel). 2021 May 26;14(6):508. doi: 10.3390/ph14060508.
4
Transition metal dichalcogenides to optimize the performance of peptide-imprinted conductive polymers as electrochemical sensors.过渡金属二硫属化物优化作为电化学传感器的肽印迹导电聚合物的性能。
Mikrochim Acta. 2021 May 27;188(6):203. doi: 10.1007/s00604-021-04850-w.
5
Accumulation of alpha-synuclein within the liver, potential role in the clearance of brain pathology associated with Parkinson's disease.肝脏中 alpha-突触核蛋白的积累，可能在清除帕金森病相关脑病理中的作用。
Acta Neuropathol Commun. 2021 Mar 20;9(1):46. doi: 10.1186/s40478-021-01136-3.
6
Cellular reprogramming with multigene activation by the delivery of CRISPR/dCas9 ribonucleoproteins via magnetic peptide-imprinted chitosan nanoparticles.通过磁性肽印迹壳聚糖纳米颗粒递送CRISPR/dCas9核糖核蛋白进行多基因激活的细胞重编程
Mater Today Bio. 2021 Jan 20;9:100091. doi: 10.1016/j.mtbio.2020.100091. eCollection 2021 Jan.
7
In situ kinetic measurements of α-synuclein aggregation reveal large population of short-lived oligomers.在体动力学测量α-突触核蛋白聚集揭示了大量短寿命寡聚物的存在。
PLoS One. 2021 Jan 22;16(1):e0245548. doi: 10.1371/journal.pone.0245548. eCollection 2021.
8
Epitope recognition of magnetic peptide-imprinted chitosan composite nanoparticles for the extraction of CRISPR/dCas9a proteins from transfected cells.磁性肽印迹壳聚糖复合纳米颗粒对抗原的识别用于从转染细胞中提取 CRISPR/dCas9a 蛋白。
Nanotechnology. 2021 Apr 30;32(18):18LT02. doi: 10.1088/1361-6528/abde00.
9
Epitope imprinting of alpha-synuclein for sensing in Parkinson's brain organoid culture medium.用于在帕金森病脑类器官培养基中进行检测的α-突触核蛋白的表位印记
Biosens Bioelectron. 2021 Mar 1;175:112852. doi: 10.1016/j.bios.2020.112852. Epub 2020 Nov 27.
10
Immunotherapy of Hepatocellular Carcinoma with Magnetic PD-1 Peptide-Imprinted Polymer Nanocomposite and Natural Killer Cells.磁性 PD-1 肽印迹聚合物纳米复合材料和自然杀伤细胞治疗肝细胞癌的免疫疗法。
Biomolecules. 2019 Oct 25;9(11):651. doi: 10.3390/biom9110651.