Baronsky Thilo, Dzementsei Aliaksandr, Oelkers Marieelen, Melchert Juliane, Pieler Tomas, Janshoff Andreas
Institute of Physical Chemistry, Tammannstr. 6, 37077 Göttingen, Germany.
The Danish Stem Cell Center, Laboratory Blegdamsvej 3b, 2200 København N, Denmark.
Integr Biol (Camb). 2016 Mar 14;8(3):349-58. doi: 10.1039/c5ib00291e.
The transition from passive to active migration of primordial germ cells in Xenopus embryos correlates with a reduction in overall adhesion to surrounding endodermal cells as well as with reduced E-cadherin expression. Single cell force spectroscopy, in which cells are brought into brief contact with a gold surface functionalized with E-cadherin constructs, allows for a quantitative estimate of functional E-cadherin molecules on the cell surface. The adhesion force between migratory PGCs and the cadherin-coated surface was almost identical to cells where E-cadherin was knocked down by morpholino oligonucleotides (180 pN). In contrast, non-migratory PGCs display significantly higher adhesion forces (270 pN) on E-cadherin functionalised surfaces. On the basis of these observations, we propose that migration of PGCs in Xenopus embryos is regulated via modulation of E-cadherin expression levels, allowing these cells to move more freely if the level of E-cadherin is reduced.
非洲爪蟾胚胎中原始生殖细胞从被动迁移到主动迁移的转变，与对周围内胚层细胞的整体黏附力降低以及E-钙黏蛋白表达减少相关。单细胞力谱技术可使细胞与用E-钙黏蛋白构建体功能化的金表面进行短暂接触，从而能够对细胞表面功能性E-钙黏蛋白分子进行定量评估。迁移的原始生殖细胞与钙黏蛋白包被表面之间的黏附力，几乎与通过吗啉代寡核苷酸敲低E-钙黏蛋白的细胞相同（180皮牛）。相比之下，非迁移性原始生殖细胞在E-钙黏蛋白功能化表面上表现出明显更高的黏附力（270皮牛）。基于这些观察结果，我们提出非洲爪蟾胚胎中原始生殖细胞的迁移是通过调节E-钙黏蛋白的表达水平来调控的，如果E-钙黏蛋白水平降低，这些细胞就能更自由地移动。
