Gary Ramla, Carbone Giovani, Petriashvili Gia, De Santo Maria Penelope, Barberi Riccardo
Physics Department, University of Calabria, Rende 87036, Italy.
Institute of Cybernetics of the Georgian Technical University, Euli str. 5, 0175 Tbilisi, Georgia.
Sensors (Basel). 2016 Feb 19;16(2):258. doi: 10.3390/s16020258.
The gold nanoparticle (GNP) aggregation growth induced by deoxyribonucleic acid (DNA) is studied by laser scanning confocal and environmental scanning electron microscopies. As in the investigated case the direct light scattering analysis is not suitable, we observe the behavior of the fluorescence produced by a dye and we detect the aggregation by the shift and the broadening of the fluorescence peak. Results of laser scanning confocal microscopy images and the fluorescence emission spectra from lambda scan mode suggest, in fact, that the intruding of the hydrophobic moiety of the probe within the cationic surfactants bilayer film coating GNPs results in a Förster resonance energy transfer. The environmental scanning electron microscopy images show that DNA molecules act as template to assemble GNPs into three-dimensional structures which are reminiscent of the DNA helix. This study is useful to design better nanobiotechnological devices using GNPs and DNA.
通过激光扫描共聚焦显微镜和环境扫描电子显微镜研究了脱氧核糖核酸（DNA）诱导的金纳米颗粒（GNP）聚集生长。在所研究的情况下，由于直接光散射分析不适用，我们观察了染料产生的荧光行为，并通过荧光峰的位移和展宽来检测聚集情况。事实上，激光扫描共聚焦显微镜图像结果以及来自λ扫描模式的荧光发射光谱表明，探针的疏水部分侵入包覆GNP的阳离子表面活性剂双层膜中会导致Förster共振能量转移。环境扫描电子显微镜图像显示，DNA分子充当模板，将GNP组装成类似于DNA螺旋的三维结构。这项研究有助于设计使用GNP和DNA的更好的纳米生物技术装置。
