Gary Ramla, Ben Salah Manel, Soltani Taoufik, Formoso Patrizia, Hbaieb Souhaira
Laboratoire de Physique de la Matière Molle et de la Modélisation Electromagnétique, Faculté des Sciences de Tunis, Université de Tunis El Manar, Rommana 1068, Tunisie.
Department of Pharmacy, Health and Nutritional Sciences, University of Calabria, 87036 Arcavacata di Rende, CS, Italy.
ACS Omega. 2024 Nov 28;9(50):49530-49538. doi: 10.1021/acsomega.4c07393. eCollection 2024 Dec 17.
This study optimizes immunofluorescence techniques using gold nanoparticles (AuNPs) to improve visualization of endothelial nitric oxide synthase (eNOS) in gill tissue. Two types of AuNP dispersions, stabilized in phosphate buffered saline (PBS) and citrate buffer (CB), were evaluated for their imaging performance. AuNPs suspended in PBS provided significantly better optical contrast due to uniform distribution and effective tissue attachment, whereas citrate-suspended AuNPs exhibited aggregation, resulting in reduced contrast. These results highlight the influence of suspension media on AuNP performance, particularly in balancing fluorescence signals to improve contrast. The PBS suspension allowed clearer visualization of eNOS, highlighting the role of AuNP compatibility in improving immunofluorescence results. This study highlights the importance of strategic selection of AuNP dispersions in contrast agent design and provides insights for advanced imaging applications where sensitivity and accurate localization of biomolecules are essential. By refining the use of AuNPs as contrast enhancers, this approach offers potential improvements in bioimaging accuracy, facilitating more precise visualization in complex tissue environments.
本研究优化了使用金纳米颗粒（AuNPs）的免疫荧光技术，以改善鳃组织中内皮型一氧化氮合酶（eNOS）的可视化。评估了两种稳定在磷酸盐缓冲盐水（PBS）和柠檬酸盐缓冲液（CB）中的AuNP分散体的成像性能。悬浮在PBS中的AuNPs由于分布均匀和有效的组织附着而提供了明显更好的光学对比度，而悬浮在柠檬酸盐中的AuNPs表现出聚集，导致对比度降低。这些结果突出了悬浮介质对AuNP性能的影响，特别是在平衡荧光信号以提高对比度方面。PBS悬浮液使eNOS的可视化更清晰，突出了AuNP兼容性在改善免疫荧光结果中的作用。本研究强调了在造影剂设计中战略性选择AuNP分散体的重要性，并为生物分子的敏感性和精确定位至关重要的先进成像应用提供了见解。通过改进AuNPs作为对比度增强剂的使用，这种方法有望提高生物成像的准确性，促进在复杂组织环境中更精确的可视化。
