Multiplexed off-axis holography using a transmission diffraction grating.

This Letter presents a novel multiplexed off-axis holographic microscope that makes use of a 1D holographic grating. The grating creates multiple object waves and projects four of them to the imaging sensor. The object waves then interfere with the reference wave at the imaging sensor, so that a multiplexed hologram is created. This approach retrieves the amplitude and phase profiles of four imaging areas with a single-exposure hologram, which greatly increases the field of view of an off-axis holographic microscope. The experimental results with the resolution target USAF 1951 T-22 and a slide of paramecia are shown to demonstrate the system's ability without sacrificing the resolution of the microscope objective.