Munir Riffat, Levin David B
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada, R3T 5V6.
Adv Biochem Eng Biotechnol. 2016;156:113-138. doi: 10.1007/10_2015_5002.
Biofuels from abundantly available cellulosic biomass are an attractive alternative to current petroleum-based fuels (fossil fuels). Although several strategies exist for commercial production of biofuels, conversion of biomass to biofuels via consolidated bioprocessing offers the potential to reduce production costs and increase processing efficiencies. In consolidated bioprocessing (CBP), enzyme production, cellulose hydrolysis, and fermentation are all carried out in a single-step by microorganisms that efficiently employ a multitude of intricate enzymes which act synergistically to breakdown cellulose and its associated cell wall components. Various strategies employed by anaerobic cellulolytic bacteria for biomass hydrolysis are described in this chapter. In addition, the regulation of CAZymes, the role of "omics" technologies in assessing lignocellulolytic ability, and current strategies for improving biomass hydrolysis for optimum biofuel production are highlighted.
由大量可得的纤维素生物质制成的生物燃料是当前石油基燃料(化石燃料)的一种有吸引力的替代品。尽管存在几种生物燃料商业化生产的策略,但通过整合生物加工将生物质转化为生物燃料有降低生产成本和提高加工效率的潜力。在整合生物加工(CBP)中,酶的产生、纤维素水解和发酵都由微生物在一步中完成,这些微生物能有效利用多种复杂的酶,这些酶协同作用以分解纤维素及其相关的细胞壁成分。本章描述了厌氧纤维素分解细菌用于生物质水解的各种策略。此外,还强调了碳水化合物活性酶的调控、“组学”技术在评估木质纤维素分解能力中的作用,以及当前为实现最佳生物燃料生产而改善生物质水解的策略。