Zhang Xuechen, Zhou Gaofeng, Shabala Sergey, Koutoulis Anthony, Shabala Lana, Johnson Peter, Li Chengdao, Zhou Meixue
School of Land and Food, University of Tasmania, P.O. Box 46, Kings Meadows, TAS, 7249, Australia.
Western Australian State Agricultural Biotechnology Centre, Murdoch University, Murdoch, Perth, WA, Australia.
Theor Appl Genet. 2016 Jun;129(6):1167-77. doi: 10.1007/s00122-016-2693-3. Epub 2016 Feb 23.
Aerenchyma formation after 7 days of waterlogging in commercial potting mixture can be a reliable, fast, and widely utilized approach for the selection of waterlogging tolerant barley genotypes. One major QTL for aerenchyma formation after 7 days of waterlogging treatment was identified and the newly developed markers explained 44 % of the phenotypic variance. This QTL can now be effectively used in barley breeding programs. Waterlogging is one of the important limiting conditions for crop yield and productivity. The main feature of waterlogged soils is oxygen deprivation, due to slow gas diffusion in water. Decreased oxygen content in waterlogged soils leads to the oxygen deficiency in plant tissues, resulting in reduced energy availability for plants. Rapidly induced aerenchyma formation is critical to maintaining adequate oxygen supply and overall waterlogging tolerance in barley. In this study, we have proved that quantifying aerenchyma formation after 7 days of waterlogging in commercial potting mixture can be a reliable, fast, and widely utilised approach for the selection of waterlogging tolerant barley genotypes, which is supported by measurements of redox potential (an indicator of anaerobic conditions). This protocol was also used to identify quantitative trait loci (QTL) in a doubled haploid population of barley from the cross between Yerong (tolerant) and Franklin (sensitive) genotypes. The QTL for aerenchyma formation and root porosity were at the same location as the waterlogging tolerance QTL. Seven new markers were developed and added onto this region on chromosome 4H. One major QTL for aerenchyma formation after 7 days waterlogging treatment explained 44.0 % of the phenotypic variance. This successful QTL for aerenchyma formation can be effectively used in the marker assisted selection to improve waterlogging tolerance in barley.
在商业盆栽混合基质中进行7天渍水后形成通气组织，对于耐渍大麦基因型的选择而言，可能是一种可靠、快速且广泛应用的方法。鉴定出了一个在渍水7天后形成通气组织的主要数量性状位点（QTL），新开发的标记解释了44%的表型变异。该QTL现在可有效地用于大麦育种计划。渍水是作物产量和生产力的重要限制条件之一。渍水土壤的主要特征是由于水中气体扩散缓慢导致氧气缺乏。渍水土壤中氧气含量降低导致植物组织缺氧，从而使植物可利用的能量减少。快速诱导通气组织形成对于维持大麦充足的氧气供应和整体耐渍性至关重要。在本研究中，我们证明了在商业盆栽混合基质中对渍水7天后的通气组织形成进行量化，对于耐渍大麦基因型的选择可能是一种可靠、快速且广泛应用的方法，氧化还原电位（厌氧条件的指标）测量结果支持了这一点。该方案还用于鉴定来自Yerong（耐渍）和Franklin（敏感）基因型杂交的大麦加倍单倍体群体中的数量性状位点（QTL）。通气组织形成和根孔隙度的QTL与耐渍性QTL位于同一位置。开发了七个新标记并添加到4H染色体的该区域。在渍水7天处理后形成通气组织的一个主要QTL解释了44.0%的表型变异。这个成功的通气组织形成QTL可有效地用于标记辅助选择，以提高大麦的耐渍性。
