School of Land and Food, University of Tasmania, Private Bag 1375, Prospect, TAS, 7250, Australia.
School of Biological Sciences, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia.
Theor Appl Genet. 2017 Aug;130(8):1559-1568. doi: 10.1007/s00122-017-2910-8. Epub 2017 Apr 26.
We report the first study on the unique allele from wild barley that can improve waterlogging tolerance in cultivated barley with a substantially higher contribution to aerenchyma formation. Waterlogging is one of the major abiotic stresses that dramatically reduce barley crop yield. Direct selection on waterlogging tolerance in the field is less effective due to its viability to environment. The most effective way of selection is to choose traits that make significant contributions to the overall tolerance and are easy to score. Aerenchyma formation under waterlogging stress is one of the most effective mechanisms to provide adequate oxygen supply and overcome stress-induced hypoxia imposed on plants. In this study, a new allele for aerenchyma formation was identified from a wild barley accession TAM407227 on chromosome 4H. Compared to that identified in cultivated barley, this allele not only produced a greater proportion of aerenchyma but made a greater contribution to the overall waterlogging tolerance. The QTL explained 76.8% of phenotypic variance in aerenchyma formation with a LOD value of 51.4. Markers co-segregating with the trait were identified and can be effectively used in marker assisted selection.
我们首次报道了来自野生大麦的独特等位基因，该等位基因可以提高栽培大麦的耐淹水能力，对通气组织形成的贡献显著更高。淹水是降低大麦作物产量的主要非生物胁迫之一。由于其对环境的生存能力，在田间直接选择耐淹水能力的效果较差。最有效的选择方法是选择对整体耐受性有重大贡献且易于评分的特征。在淹水胁迫下通气组织的形成是为植物提供充足氧气供应并克服胁迫诱导缺氧的最有效机制之一。在这项研究中，从野生大麦 TAM407227 品系中鉴定到一个新的通气组织形成等位基因，位于 4H 染色体上。与在栽培大麦中鉴定到的等位基因相比，该等位基因不仅产生了更大比例的通气组织，而且对整体耐淹水能力的贡献更大。该 QTL 解释了通气组织形成表型方差的 76.8%，LOD 值为 51.4。与该性状共分离的标记已被鉴定出来，并可有效用于标记辅助选择。
