Feng Guang-Da, Yang Song-Zhen, Xiong Xiong, Li Hua-Ping, Zhu Hong-Hui
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology,Guangzhou 510070, PRChina.
College of Agriculture, South China Agricultural University,Guangzhou 510642, PRChina.
Int J Syst Evol Microbiol. 2016 May;66(5):2046-2051. doi: 10.1099/ijsem.0.000989. Epub 2016 Feb 23.
A Gram-stain-negative and non-motile bacterial strain designated 9O-5T was isolated from an abandoned lead-zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5T belongs to the genus Sphingomonas and was closely related to Sphingomonas abaci DSM 15867T (97.6 % similarity), Sphingomonas phyllosphaerae FA2T (96.9 %) and Shingomonas guangdongensis 9NM-8T (96.8 %). Mean DNA-DNA relatedness between strain 9O-5T and S. abaci DSM 15867T was only 47.1 ± 4.9 %. The major fatty acids were C18 : 1ω7c, C14 : 0 2-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). It contained Q-10 as the predominant respiratory quinone and sym-homospermidine as the major polyamine. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids and six unidentified lipids. The genomic DNA G+C content of strain 9O-5T was 69.1 ± 0.1 mol%. Based on the data from this polyphasic taxonomic study, strain 9O-5T should be considered as representing a novel species of the genus Sphingomonas, for which the name Sphingomonas metalli sp. nov. is proposed. The type strain is 9O-5T ( = CGMCC 1.15330T = KCTC 42759T).
从中国南方广东省梅州市的一个废弃铅锌矿中分离出一株革兰氏染色阴性且不运动的细菌菌株，命名为9O-5T。该分离株呈橙色色素，好氧，氧化酶和过氧化氢酶呈阳性。基于16S rRNA基因序列的系统发育分析表明，菌株9O-5T属于鞘氨醇单胞菌属，与阿氏鞘氨醇单胞菌DSM 15867T（相似度97.6%）、叶鞘氨醇单胞菌FA2T（96.9%）和广东鞘氨醇单胞菌9NM-8T（96.8%）密切相关。菌株9O-5T与阿氏鞘氨醇单胞菌DSM 15867T之间的平均DNA-DNA相关性仅为47.1±4.9%。主要脂肪酸为C18:1ω7c、C14:0 2-OH和总和特征3（C16:1ω7c和/或C16:1ω6c）。它含有Q-10作为主要呼吸醌，对称高亚精胺作为主要多胺。极性脂质为鞘糖脂、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、五种未鉴定的磷脂和六种未鉴定的脂质。菌株9O-5T的基因组DNA G+C含量为69.1±0.1 mol%。基于这项多相分类研究的数据，菌株9O-5T应被视为代表鞘氨醇单胞菌属的一个新物种，为此提出了新名称金属鞘氨醇单胞菌（Sphingomonas metalli sp. nov.）。模式菌株为9O-5T（=CGMCC 1.15330T=KCTC 42759T）。
