Fan Qiu-Ming, Zhang Ren-Gang, Chen Han-Yi, Feng Qing-Qing, Lv Jie
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Int J Syst Evol Microbiol. 2019 Jun;69(6):1531-1536. doi: 10.1099/ijsem.0.002983. Epub 2018 Sep 11.
A Gram-stain-negative, non-motile, non-sporulating, rod-shaped, orange-pigmented bacterium, designated strain FQM01, was isolated from a subterranean sediment sample in the Mohe permafrost area, China. Strain FQM01 grew optimally at 25 °C, pH 7.0 and NaCl concentration of 0 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FQM01 belonged to the genus Sphingomonas. The closest phylogenetic relative was Sphingomonas spermidinifaciens GDMCC 1.657 (97.6 %), followed by Sphingomonas mucosissima DSM 17494 (97.2 %). The DNA G+C content of the isolate was 66.9 mol%. Strain FQM01 contained Q-10 as the predominant ubiquinone, and C18 : 1ω6c and/or C18 : 1ω7c, C16 : 1ω6c and/or C16 : 1ω7c, C16 : 0, C14 : 0 2-OH and C18 : 1ω7c 11 methyl as the major fatty acids. Major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and an unidentified glycolipid. Only sym-homospermidine was detected as the polyamine. On the basis of phylogenetic and phenotypic data, strain FQM01 is considered to represent a novel species of Sphingomonas for which the name Sphingomonasfloccifaciens sp. nov. is proposed. The type strain is FQM01 (=CGMCC 1.15797=KCTC 52630).
从中国漠河北极冻土区的地下沉积物样本中分离出一株革兰氏阴性、无运动性、不产芽孢、杆状、产橙色色素的细菌，命名为FQM01菌株。FQM01菌株在25°C、pH 7.0和NaCl浓度为0%（w/v）的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，FQM01菌株属于鞘氨醇单胞菌属。最接近的系统发育亲缘种是产亚精胺鞘氨醇单胞菌GDMCC 1.657（相似度97.6%），其次是粘质鞘氨醇单胞菌DSM 17494（相似度97.2%）。该分离株的DNA G+C含量为66.9 mol%。FQM01菌株以Q-10作为主要泛醌，以C18:1ω6c和/或C18:1ω7c、C16:1ω6c和/或C16:1ω7c、C16:0、C14:0 2-OH和C18:1ω7c 11-甲基作为主要脂肪酸。主要极性脂为磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、鞘糖脂和一种未鉴定的糖脂。仅检测到对称高亚精胺作为多胺。基于系统发育和表型数据，FQM01菌株被认为代表鞘氨醇单胞菌属的一个新物种，为此提出新名称絮凝鞘氨醇单胞菌（Sphingomonas floccifaciens sp. nov.）。模式菌株为FQM01（=CGMCC 1.15797=KCTC 52630）。
