Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, UK.
Fungal Genet Biol. 2011 Sep;48(9):849-57. doi: 10.1016/j.fgb.2011.02.004. Epub 2011 Mar 22.
Fungal hyphae show extreme polarized growth at the tip. Electron microscope studies have revealed a apical body called the Spitzenkörper that is thought to drive polarized growth. Studies of polarized growth in S. cerevisiae have identified the protein components of the polarized growth machinery, that are conserved in other fungi. Fusion of these proteins to GFP and its variants has for the first time allowed the localization of these proteins in real time to the hyphal tip without the need for drastic fixation procedures. Such studies showed that vesicle-associated proteins localize to the Spitzenkörper and identified a second compartment located at the tip surface composed of exocyst and other proteins that mediate the fusion of secretory vesicles with the plasma membrane.
真菌菌丝在顶端表现出极端的极化生长。电子显微镜研究揭示了一个称为顶端体的顶端体，它被认为驱动极化生长。对酿酒酵母中极化生长的研究已经确定了极化生长机制的蛋白质成分，这些成分在其他真菌中是保守的。这些蛋白质与 GFP 及其变体融合，首次允许在不需要剧烈固定程序的情况下实时将这些蛋白质定位到菌丝顶端。这些研究表明，囊泡相关蛋白定位于顶端体，并鉴定出位于顶端表面的第二个隔室，该隔室由外泌体和其他介导分泌囊泡与质膜融合的蛋白质组成。
