Hofbauer Anna, Melnik Stanislav, Tschofen Marc, Arcalis Elsa, Phan Hoang T, Gresch Ulrike, Lampel Johannes, Conrad Udo, Stoger Eva
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences Vienna, Austria.
Department of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben, Germany.
Front Plant Sci. 2016 Feb 16;7:142. doi: 10.3389/fpls.2016.00142. eCollection 2016.
Zein is a water-insoluble polymer from maize seeds that has been widely used to produce carrier particles for the delivery of therapeutic molecules. We encapsulated a recombinant model vaccine antigen in newly formed zein bodies in planta by generating a fusion construct comprising the ectodomain of hemagglutinin subtype 5 and the N-terminal part of γ-zein. The chimeric protein was transiently produced in tobacco leaves, and H5-containing protein bodies (PBs) were used to immunize mice. An immune response was achieved in all mice treated with H5-zein, even at low doses. The fusion to zein markedly enhanced the IgG response compared the soluble H5 control, and the effect was similar to a commercial adjuvant. The co-administration of adjuvants with the H5-zein bodies did not enhance the immune response any further, suggesting that the zein portion itself mediates an adjuvant effect. While the zein portion used to induce protein body formation was only weakly immunogenic, our results indicate that zein-induced PBs are promising production and delivery vehicles for subunit vaccines.
玉米醇溶蛋白是一种来自玉米种子的水不溶性聚合物，已被广泛用于生产用于递送治疗性分子的载体颗粒。我们通过构建一种融合构建体，将重组模型疫苗抗原包裹在植物中新形成的玉米醇溶蛋白体中，该融合构建体包含血凝素5亚型的胞外域和γ-玉米醇溶蛋白的N端部分。嵌合蛋白在烟草叶片中瞬时产生，含H5的蛋白体（PBs）用于免疫小鼠。即使在低剂量下，所有用H5-玉米醇溶蛋白处理的小鼠都产生了免疫反应。与可溶性H5对照相比，与玉米醇溶蛋白的融合显著增强了IgG反应，其效果与商业佐剂相似。佐剂与H5-玉米醇溶蛋白体共同给药并未进一步增强免疫反应，这表明玉米醇溶蛋白部分本身介导了佐剂效应。虽然用于诱导蛋白体形成的玉米醇溶蛋白部分免疫原性较弱，但我们的结果表明，玉米醇溶蛋白诱导的PBs是用于亚单位疫苗的有前景的生产和递送载体。
