Li Yongwen, Li Ying, Liu Jinghao, Fan Yaguang, Li Xin, Dong Ming, Liu Hongyu, Chen Jun
b Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment , Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital , Tianjin , China.
a Department of Lung Cancer Surgery , Tianjin Medical University General Hospital , Tianjin , China.
Cancer Biol Ther. 2016;17(3):272-9. doi: 10.1080/15384047.2016.1139242. Epub 2016 Jan 30.
Although metastasis remains the overwhelming cause of death for patients with non-small cell lung cancer (NSCLC), the underlying mechanisms of metastasis remain unknown. Accumulating evidence suggests that microRNAs (miRNAs) are key players in the regulation of tumor cell invasion and metastasis. Expression of miR-9, miR-10b, miR-145, and miR-155, 4 miRNAs previously shown to play roles in metastasis in other tumor types, was compared in lymph node (LN)-positive NSCLC versus LN-negative NSCLC. Expression of miR-145 was significantly lower in LN-positive NSCLC (P < 0.05), while expression of miR-10b was significantly higher (P < 0.05). Expression of both miR-145 and miR-10b was correlated with lymph node metastasis in NSCLC (both Ps < 0.001). In addition, miR-10b facilitated the migration and invasion of lung cancer cell line A549, while miR-145 suppressed the migration and invasion capacity of A549 in vitro. These results suggest that miR-10b and miR-145 may act as an oncogene or tumor suppressor gene, respectively, in NSCLC metastasis.
尽管转移仍是非小细胞肺癌（NSCLC）患者死亡的主要原因，但转移的潜在机制仍不清楚。越来越多的证据表明，微小RNA（miRNA）是肿瘤细胞侵袭和转移调控中的关键因子。比较了先前显示在其他肿瘤类型转移中起作用的4种miRNA，即miR-9、miR-10b、miR-145和miR-155在淋巴结（LN）阳性NSCLC与LN阴性NSCLC中的表达。miR-145在LN阳性NSCLC中的表达显著降低（P < 0.05），而miR-10b的表达显著升高（P < 0.05）。miR-145和miR-10b的表达均与NSCLC中的淋巴结转移相关（P均< 0.001）。此外，miR-10b促进肺癌细胞系A549的迁移和侵袭，而miR-145在体外抑制A549的迁移和侵袭能力。这些结果表明，miR-10b和miR-145在NSCLC转移中可能分别作为癌基因或肿瘤抑制基因发挥作用。
