Palmer S, Wakelam M J
Department of Biochemistry, University of Glasgow, U.K.
Biochim Biophys Acta. 1989 Dec 14;1014(3):239-46. doi: 10.1016/0167-4889(89)90219-x.
This review summarises the methods available for the mass measurement of inositol phosphates, i.e., use of radioactive inositol lipid precursors, optical techniques, gas chromatography, mass spectrometry, nuclear magnetic resonance, fast atom bombardment and assays specific for Ins(1,4,5)P3. Examples of the use of each method, its sensitivity, advantages and drawbacks are given.
本综述总结了可用于肌醇磷酸大规模测量的方法，即使用放射性肌醇脂质前体、光学技术、气相色谱法、质谱法、核磁共振法、快原子轰击法以及针对Ins(1,4,5)P3的特异性测定法。文中给出了每种方法的使用示例、灵敏度、优点和缺点。
