Boodhoo Nitish, Sharif Shayan, Behboudi Shahriar
The Pirbright Institute, Ash Road, Woking, United Kingdom.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
PLoS One. 2016 Feb 24;11(2):e0150134. doi: 10.1371/journal.pone.0150134. eCollection 2016.
1,25-Dihydroxyvitamin D3 (Vitamin D) is a naturally synthesized fat soluble vitamin shown to have immunomodulatory, anti-inflammatory and cancer prevention properties in human and murine models. Here, we studied the effects of Vitamin D on the functional abilities of avian T lymphocytes using chicken Interferon (IFN)-γ ELISPOT assay, BrdU proliferation assay, Annexin V apoptosis assay and PhosFlow for detecting phosphorylated signalling molecules. The results demonstrate that Vitamin D significantly inhibited the abilities of T lymphocytes to produce IFN-γ and proliferate in vitro (P≤0.05), but retained their ability to undergo degranulation, which is a maker for cytotoxicity of these cells. Similarly, Vitamin D did not inhibit Extracellular signal-Regulated Kinase (ERK) 1/2 phosphorylation, a key mediator in T cell signalling, in the stimulated T lymphocytes population, while reduced ERK1/2 phosphorylation levels in the unstimulated cells. Our data provide evidence that Vitamin D has immuno-modulatory properties on chicken T lymphocytes without inducing unresponsiveness and by limiting immuno-pathology can promote protective immunity against infectious diseases of poultry.
1,25 - 二羟基维生素D3（维生素D）是一种天然合成的脂溶性维生素，在人和小鼠模型中已显示出具有免疫调节、抗炎和防癌特性。在此，我们使用鸡干扰素（IFN）-γ酶联免疫斑点分析、BrdU增殖分析、膜联蛋白V凋亡分析以及用于检测磷酸化信号分子的磷酸化流式细胞术，研究了维生素D对禽类T淋巴细胞功能能力的影响。结果表明，维生素D显著抑制T淋巴细胞在体外产生IFN - γ和增殖的能力（P≤0.05），但保留了它们进行脱颗粒的能力，脱颗粒是这些细胞细胞毒性的一个标志。同样，维生素D在受刺激的T淋巴细胞群体中并未抑制细胞外信号调节激酶（ERK）1/2的磷酸化，ERK1/2磷酸化是T细胞信号传导中的关键介质，而在未受刺激的细胞中降低了ERK1/2的磷酸化水平。我们的数据提供了证据，表明维生素D对鸡T淋巴细胞具有免疫调节特性，不会诱导无反应性，并且通过限制免疫病理学可促进针对家禽传染病的保护性免疫。
