Cvrčková Fatima, Oulehlová Denisa, Žárský Viktor
a Department of Experimental Plant Biology , Faculty of Sciences, Charles University , Prague , Czech Republic.
Plant Signal Behav. 2016;11(3):e1155017. doi: 10.1080/15592324.2016.1155017.
Development of the plant aerial organs epidermis involves a complex interplay of cytoskeletal rearrangements, membrane trafficking-dependent cell surface expansion, and intra- and intercellular signaling, resulting in a pattern of perfectly interlocking pavement cells. While recent detailed in vivo observations convincingly identify microtubules rather than actin as key players at the early stages of development of pavement cell lobes in Arabidopsis, mutations affecting the actin-nucleating ARP2/3 complex are long known to reduce pavement cell lobing, suggesting a central role for actin. We have now shown that functional impairment of the Arabidopsis formin FH1 enhances both microtubule dynamics and pavement cell lobing. While formins are best known for their ability to nucleate actin, many members of this old gene family now emerge as direct or indirect regulators of the microtubule cytoskeleton, and our findings suggest that they might co-ordinate action of the two cytoskeletal systems during pavement cell morphogenesis.
植物地上器官表皮的发育涉及细胞骨架重排、依赖膜泡运输的细胞表面扩张以及细胞内和细胞间信号传导之间的复杂相互作用，最终形成紧密联锁的扁平细胞模式。虽然最近的详细体内观察令人信服地表明，在拟南芥扁平细胞叶发育的早期阶段，起关键作用的是微管而非肌动蛋白，但长期以来人们已知影响肌动蛋白成核ARP2/3复合体的突变会减少扁平细胞叶的形成，这表明肌动蛋白起核心作用。我们现在已经表明，拟南芥formin FH1的功能受损会增强微管动力学和扁平细胞叶的形成。虽然formin以其使肌动蛋白成核的能力而最为人所知，但这个古老基因家族的许多成员现在已成为微管细胞骨架的直接或间接调节因子，我们的研究结果表明，它们可能在扁平细胞形态发生过程中协调两个细胞骨架系统的作用。
