Li Xin, Qiao Yan, Zhong Guo-zhen
Huan Jing Ke Xue. 2015 Nov;36(11):4319-24.
Polycyclic aromatic hydrocarbons (PAHs) are common environmental pollutants which are concerned because several of its members have been known to be carcinogenic and mutagenic. Pyrene, as a kind of PAHs, was covalently linked to carrier protein bovine serum albumin by active ester method and immunized to mice. A sensitive mAb 6A6 was developed by a three-step screening procedure. The IC50 of 6 A6 against pyrene and benzo(a) pyrene were 8.1 μg x L(-1) and 6.8 μg x L(-1), respectively. The cross-reactivity values (CR) with other PAHs were less than 5%. An immunosensor was developed based on 6A6 for pyrene and benzo(a) pyrene detection with a linear range of 0.2-10.0 μg x L(-1). The assessment of spiked water samples by the sensor demonstrated faster and more sensitive than ELISA method. We believe that this sensor can be used to rapidly assess pyrene and benzo(a) pyrene in environmental samples.
多环芳烃（PAHs）是常见的环境污染物，因其部分成员具有致癌性和致突变性而备受关注。芘作为多环芳烃的一种，通过活性酯法与载体蛋白牛血清白蛋白共价连接，并免疫小鼠。通过三步筛选程序获得了一种灵敏的单克隆抗体6A6。6A6对芘和苯并(a)芘的半数抑制浓度（IC50）分别为8.1 μg x L(-1)和6.8 μg x L(-1)。与其他多环芳烃的交叉反应值（CR）小于5%。基于6A6开发了一种用于检测芘和苯并(a)芘的免疫传感器，线性范围为0.2 - 10.0 μg x L(-1)。该传感器对加标水样的评估表明，其比酶联免疫吸附测定（ELISA）方法更快、更灵敏。我们相信该传感器可用于快速评估环境样品中的芘和苯并(a)芘。
