Hess M, Raue R, Prusas C
a Institut für Geflügelkrankheiten , Freie Universität Berlin , Koserstraβe 21 , Berlin , 14195 , Germany.
Avian Pathol. 1999 Oct;28(5):433-9. doi: 10.1080/03079459994443.
Serology, restriction enzyme analysis and polymerase chain reactions were used to classify a total of 12 fowl adenoviruses (FAV) isolated from clinical cases of infectious hydropericardium from field outbreaks in seven countries in Asia and America. All isolates belonged to FAV serotype 4. Two isolates were contaminated with avian adeno-associated virus and one of them also contained FAV1. Minor differences were observed in the BamHI restriction profiles. More variability was seen with SmaI, BglII and PstI restriction profiles. However, more than 80% of the fragments were identical in size in the five different PstI profiles, indicating the close genomic relationship between the isolates. Polymerase chain reaction assays supported the classification of the isolates as FAV4 strains. All isolates could be detected using H1/H2 or H3/H4 primer pairs. Restriction enzyme analysis of the H1/H2 polymerase chain reaction (PCR) products allowed no differentiation between the isolates, whereas the three isolates from India and Pakistan could be separated from all others after HpaII digestion of the H3/H4 PCR products. Although strain variation was demonstrated, it could be shown that all adenoviruses isolated from various field cases of infectious hydropericardium (Angara Disease) in several countries belong to fowl adenovirus serotype 4.
采用血清学、限制性内切酶分析和聚合酶链反应对从亚洲和美洲7个国家野外暴发的传染性心包积水临床病例中分离出的12株禽腺病毒（FAV）进行分类。所有分离株均属于FAV血清型4。两株分离株被禽腺相关病毒污染，其中一株还含有FAV1。在BamHI限制性酶切图谱中观察到微小差异。SmaI、BglII和PstI限制性酶切图谱显示出更多变异性。然而，在5种不同的PstI图谱中，超过80%的片段大小相同，表明分离株之间存在密切的基因组关系。聚合酶链反应分析支持将分离株分类为FAV4株。使用H1/H2或H3/H4引物对可检测到所有分离株。对H1/H2聚合酶链反应（PCR）产物进行限制性酶切分析无法区分分离株，而对来自印度和巴基斯坦的3株分离株的H3/H4 PCR产物进行HpaII消化后，可将其与所有其他分离株区分开来。尽管显示出菌株变异，但可以表明，从几个国家各种传染性心包积水（安加拉病）野外病例中分离出的所有腺病毒均属于禽腺病毒血清型4。
