Dahiya Swati, Srivastava Ravinder N, Hess Michael, Gulati Baldev R
Department of Veterinary Microbiology, CCS Haryana Agricultural University, Hisar, India.
Avian Dis. 2002 Jan-Mar;46(1):230-3. doi: 10.1637/0005-2086(2002)046[0230:FASAWO]2.0.CO;2.
During 1998, hydropericardium syndrome was observed among 3-to-6-wk-old broilers in 45 different flocks of Haryana, India, with mortality ranging between 10% and 30%. Fowl adenovirus (FAV) was isolated from one of the affected flocks by chicken embryo liver cell culture. Serum neutralization test and polymerase chain reaction assay coupled with restriction enzyme analysis confirmed that the isolated virus belonged to FAV serotype 4. The disease was reproduced in 28-day-old broilers by subcutaneous and oral inoculation of isolated FAV4 alone. Typical hydropericardium and basophilic intranuclear inclusions in hepatocytes were observed in experimental birds by day 4 postinoculation.
1998年期间，在印度哈里亚纳邦45个不同鸡群的3至6周龄肉鸡中观察到心包积水综合征，死亡率在10%至30%之间。通过鸡胚肝细胞培养从其中一个受影响鸡群中分离出禽腺病毒（FAV）。血清中和试验以及聚合酶链反应检测结合限制性内切酶分析证实，分离出的病毒属于FAV血清型4。通过单独皮下和口服接种分离出的FAV4，在28日龄肉鸡中复制出了该病。接种后第4天，在实验鸡中观察到典型的心包积水和肝细胞中的嗜碱性核内包涵体。
