炎症性成牙本质细胞凋亡增加与CD47缺失有关。
Increased Apoptosis of Inflamed Odontoblasts Is Associated with CD47 Loss.
作者信息
Wang H S, Pei F, Chen Z, Zhang L
机构信息
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOS and Key Laboratory for Oral Biomedicine of Ministry of Education [KLOBM]), School and Hospital of Stomatology, Wuhan University, Wuhan, China Department of Endodontics, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
出版信息
J Dent Res. 2016 Jun;95(6):697-703. doi: 10.1177/0022034516633639. Epub 2016 Feb 24.
Survival of odontoblasts during infection and inflammation determines prognosis of the dental pulp. CD47 is a "self"-label surface marker on viable cells. The aim of the present study is to investigate the interaction between CD47, autophagy, and apoptosis in inflamed human dental pulp and lipopolysaccharide (LPS)-treated mDPC6T cells. We identified activation of autophagy and apoptosis in the odontoblasts due to inflammation of the human dental pulp. Furthermore, downregulation of CD47 correlated with increased autophagy and apoptosis in dental pulp of the patients afflicted with either caries and/or pulpitis. We also detected colocalization of CD47 and LC3 in the inflamed odontoblasts. In addition, functional study indicated that loss of CD47 activates autophagy and increases apoptosis, indicating that CD47 plays a key role in the LPS-induced autophagy and apoptosis of odontoblasts.
成牙本质细胞在感染和炎症期间的存活决定牙髓的预后。CD47是活细胞上的一种“自我”标记表面分子。本研究的目的是探讨CD47、自噬和凋亡在人炎症牙髓及脂多糖（LPS）处理的小鼠牙髓细胞（mDPC6T）中的相互作用。我们发现，人牙髓炎症可导致成牙本质细胞自噬和凋亡激活。此外，在患有龋齿和/或牙髓炎的患者牙髓中，CD47的下调与自噬和凋亡增加相关。我们还检测到炎症成牙本质细胞中CD47与微管相关蛋白1轻链3（LC3）的共定位。此外，功能研究表明，CD47缺失可激活自噬并增加凋亡，表明CD47在LPS诱导的成牙本质细胞自噬和凋亡中起关键作用。
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