Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand; Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Department of Restorative Dentistry and Periodontology, Faculty of Dentistry, Chiang Mai University, Chiang Mai, Thailand.
Eur Endod J. 2023 Mar;8(2):148-155. doi: 10.14744/eej.2022.74745.
Mitochondrial dynamics play a pivotal role in maintaining the homeostasis of the dental pulp. Inflammation and oxidative stress can trigger changes in mitochondrial dynamics, leading to cell death in the dental pulp. This study aimed to investigate inflammation, oxidative stress, mitochondrial dynamic alterations, and cell death in inflamed pulpal tissues compared to healthy pulp tissues.
Pulpal tissues were collected (n=15 per group) from: 1) healthy people as the control and 2) people with clinically diagnosed irreversible pulpitis. Proteins indicating inflammation, oxidative stress, mitochondrial dynamics, and cell death markers were investigated by western blot analysis. A Student's t-test was used to analyse differences between the healthy and irreversible pulpitis groups. A probability of 0.05 was used to indicate statistical significance (p<0.05).
The expression of the proteins, tumour necrosis factor-alpha (TNF-α) and nuclear factor kappa-lightchain-enhancer, by activated B cells (NF-κB) from inflamed pulp tissues were significantly higher than those of control. Compared to controls, 4 hydroxynonenal (4HNE) and dynamin-related protein 1 (Drp1) were significantly higher, while mitofusin 2 (MFN2) and optic atrophy type 1 (OPA1) were significantly lower in inflamed pulp tissues. Bcl-2-associated X protein (Bax), cleaved caspase-3, and cytochrome c were significantly higher in inflamed pulpal tissues compared to controls. In inflamed pulpal tissues, we found a significant increase in the expression of receptor-interacting serine or threonine-protein kinase 1 (RIPK1) but not receptor-interacting serine or threonine-protein kinase 3 (RIPK3).
Irreversible pulpitis is associated with inflammation, oxidative stress, alterations in mitochondrial dynamics, and apoptosis in pulpal tissues. (EEJ-2022-01-014).
线粒体动力学在维持牙髓内环境稳定中起着关键作用。炎症和氧化应激可引发线粒体动力学变化，导致牙髓细胞死亡。本研究旨在比较炎症牙髓组织与健康牙髓组织，探讨炎症、氧化应激、线粒体动力学改变和细胞死亡。
收集（每组 15 例）来自：1）健康人作为对照和 2）临床诊断为不可复性牙髓炎的人的牙髓组织。通过 Western blot 分析研究炎症、氧化应激、线粒体动力学和细胞死亡标志物的蛋白表达。采用 Student's t 检验比较健康和不可复性牙髓炎组之间的差异。概率为 0.05 表示具有统计学意义（p<0.05）。
与对照组相比，来自炎症牙髓组织的激活 B 细胞的肿瘤坏死因子-α（TNF-α）和核因子 kappa 轻链增强子（NF-κB）的蛋白表达显著升高。与对照组相比，4 羟基壬烯醛（4HNE）和动力相关蛋白 1（Drp1）在炎症牙髓组织中显著升高，而线粒体融合蛋白 2（MFN2）和视神经萎缩 1 型（OPA1）在炎症牙髓组织中显著降低。与对照组相比，Bcl-2 相关 X 蛋白（Bax）、裂解的半胱天冬酶 3 和细胞色素 c 在炎症牙髓组织中表达显著升高。在炎症牙髓组织中，我们发现受体相互作用丝氨酸/苏氨酸蛋白激酶 1（RIPK1）的表达显著增加，但受体相互作用丝氨酸/苏氨酸蛋白激酶 3（RIPK3）的表达没有增加。
不可复性牙髓炎与牙髓组织中的炎症、氧化应激、线粒体动力学改变和细胞凋亡有关。（EEJ-2022-01-014）。
