Andersson Patiyan, Harris Simon R, Smith Helena M B Seth, Hadfield James, O'Neill Colette, Cutcliffe Lesley T, Douglas Fiona P, Asche L Valerie, Mathews John D, Hutton Susan I, Sarovich Derek S, Tong Steven Y C, Clarke Ian N, Thomson Nicholas R, Giffard Philip M
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Casuarina, Northern Territory 0811, Australia.
Pathogen Variation Programme, The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge CB10 1SA, UK.
Nat Commun. 2016 Feb 25;7:10688. doi: 10.1038/ncomms10688.
Chlamydia trachomatis causes sexually transmitted infections and the blinding disease trachoma. Current data on C. trachomatis phylogeny show that there is only a single trachoma-causing clade, which is distinct from the lineages causing urogenital tract (UGT) and lymphogranuloma venerum diseases. Here we report the whole-genome sequences of ocular C. trachomatis isolates obtained from young children with clinical signs of trachoma in a trachoma endemic region of northern Australia. The isolates form two lineages that fall outside the classical trachoma lineage, instead being placed within UGT clades of the C. trachomatis phylogenetic tree. The Australian trachoma isolates appear to be recombinants with UGT C. trachomatis genome backbones, in which loci that encode immunodominant surface proteins (ompA and pmpEFGH) have been replaced by those characteristic of classical ocular isolates. This suggests that ocular tropism and association with trachoma are functionally associated with some sequence variants of ompA and pmpEFGH.
沙眼衣原体可引发性传播感染以及致盲疾病沙眼。目前关于沙眼衣原体系统发育的资料显示，只有一个导致沙眼的进化枝，它与引发泌尿生殖道（UGT）疾病和性病性淋巴肉芽肿的谱系不同。在此，我们报告了从澳大利亚北部沙眼流行地区患有沙眼临床症状的幼儿中分离出的眼用沙眼衣原体菌株的全基因组序列。这些分离株形成了两个进化枝，它们不属于经典的沙眼进化枝，而是位于沙眼衣原体系统发育树的UGT进化枝内。澳大利亚的沙眼分离株似乎是与UGT沙眼衣原体基因组主干的重组体，其中编码免疫显性表面蛋白（ompA和pmpEFGH）的基因座已被经典眼用分离株的特征性基因座所取代。这表明眼嗜性以及与沙眼的关联在功能上与ompA和pmpEFGH的某些序列变异有关。
