Sturtevant J M, Mateo P L
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2584-7. doi: 10.1073/pnas.75.6.2584.
A number of authors have reported observations on D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3.] that they have interpreted in terms of a temperature-dependent conformational transition having a van't Hoff enthalpy amounting to more than 1 cal per g of protein (1 cal = 4.184J). No indication of this transition is obtained by using a differential scanning calorimeter having a sensitivity considerably in excess of that required to detect such a transition. The implications of this discrepancy are discussed.
许多作者报告了关于D-氨基酸氧化酶[D-氨基酸:O2氧化还原酶(脱氨基),EC 1.4.3.3.]的观察结果,他们将其解释为温度依赖性构象转变,其范特霍夫焓超过每克蛋白质1卡(1卡 = 4.184焦耳)。使用灵敏度远高于检测这种转变所需灵敏度的差示扫描量热计,未获得这种转变的迹象。讨论了这种差异的影响。