Otsuki K, Ito T, Kenmochi T, Maruyama M, Akutsu N, Saigo K, Hasegawa M, Aoyama H, Matsumoto I, Uchino Y
Department of Surgery, National Chiba-East Hospital, Chiba, Japan.
Department of Transplantation Surgery, National Chiba-East Hospital, Chiba, Japan.
Transplant Proc. 2016 Jan-Feb;48(1):229-33. doi: 10.1016/j.transproceed.2015.12.025.
The use of positron-emission tomography (PET) with (18)F-fluorodeoxyglucose (FDG) -labeled islets has been considered to be a potential modality to visualize and quantify early engraftment of islet transplantation. The objective of this study was to evaluate the early islets' survival of the FDG-labeled islets with or without warm ischemic stress in portal transplanted rats using PET and autoradiography.
Islets were isolated from Lewis rat pancreata with or without 30-minute warm ischemia times (WITs). For islets' labeling, 300 islets were incubated with 3 MBq FDG for 60 minutes. FDG-labeled islets were transplanted into the liver via portal vein. In in vivo study, a PET study was scanned for 90 minutes and the FDG uptake was expressed as percentage of liver injection dose (ID). In ex vivo study, the liver was exposed for 30 minutes with single fluorescence autoradiography.
In the PET study, the percentage of liver ID of the islets without WIT was 27.8 and that of the WIT islets was 20.1 at the end of islet transplantation. At 90 minutes after transplantation, the percentage of liver ID was decreased to 14.7 in the islets without WIT and 10.1 in the WIT islets. In the autoradiogram, the number of hot spots was more obviously visualized in the liver transplanted without WIT islets than in the liver transplanted with WIT islets.
Almost 50% of the islets were immediately lost in both the islets without WIT and those with WIT transplantation in the early period. However, islet survival was 1.4 times higher in the islets without WIT than that in those with WIT in the early engraftment phase.
使用正电子发射断层扫描（PET）结合（18）F-氟脱氧葡萄糖（FDG）标记的胰岛被认为是一种可视化和量化胰岛移植早期植入情况的潜在方法。本研究的目的是利用PET和放射自显影技术评估门静脉移植大鼠中，有或无热缺血应激情况下FDG标记胰岛的早期存活情况。
从Lewis大鼠胰腺中分离胰岛，热缺血时间（WIT）为30分钟或无热缺血。为标记胰岛，将300个胰岛与3 MBq FDG孵育60分钟。将FDG标记的胰岛经门静脉移植到肝脏。在体内研究中，进行90分钟的PET扫描，FDG摄取量以肝脏注射剂量（ID）的百分比表示。在体外研究中，将肝脏暴露30分钟进行单荧光放射自显影。
在PET研究中，胰岛移植结束时，无WIT的胰岛肝脏ID百分比为27.8，有WIT的胰岛为20.1。移植后90分钟，无WIT的胰岛肝脏ID百分比降至14.7，有WIT的胰岛降至10.1。在放射自显影片中，无WIT胰岛移植的肝脏中热点数量比有WIT胰岛移植的肝脏中更明显可见。
在早期，无WIT和有WIT移植的胰岛中几乎有50%立即丢失。然而，在早期植入阶段，无WIT的胰岛存活率比有WIT的胰岛高1.4倍。
