评估18F-FDG白细胞成像监测胰岛移植后排斥反应的情况。
Assessment of 18F-FDG-leukocyte imaging to monitor rejection after pancreatic islet transplantation.
作者信息
Toso C, Zaidi H, Morel P, Armanet M, Wojtusciszyn A, Mai G, Baertschiger R, Buhler L, Berney T
机构信息
Cell Isolation and Transplantation Center, Clinic for Visceral and Transplant Surgery, University Hospital, Geneva, Switzerland.
出版信息
Transplant Proc. 2006 Nov;38(9):3033-4. doi: 10.1016/j.transproceed.2006.08.135.
AIM
We sought to investigate the feasibility of 18F-FDG-leukocyte imaging to detect islet rejection.
METHODS
Two thousand Sprague-Dawley (SD, syngeneic group) or Lewis (allogeneic group) islet equivalents were intraportally injected into SD rat recipients. Four and 7 days after transplantation, 10(8) 18F-FDG-labeled splenocytes were injected into the jugular vein. Splenocytes were harvested from naïve or sensitized (12 days after intraportal transplantation of 2000 Lewis IEQ) SD rats. Positron emission tomography (PET) imaging was started 5 minutes after splenocyte infusion and performed hourly for 4 hours.
RESULTS
One hour after splenocyte injection, FDG was mainly detected in the heart and lungs. It was then further distributed to other organs, and from the second hour, the highest tracer concentration was located in the abdomen. Liver FDG uptake was similar between syngeneic, allogeneic, and sensitized allogeneic groups at 4 and 7 days after islet transplantation.
DISCUSSION
No islet rejection was detected by 18F-FDG-leukocyte imaging. The amount of transplanted tissue was only few millilitres and the additional related inflammation in case of rejection is small and difficult to detect. The liver showed a relatively high spontaneous tracer uptake; the related background prevented detection of a potential increase in tracer uptake in cases of islet rejection.
目的
我们试图研究18F-FDG-白细胞成像检测胰岛排斥反应的可行性。
方法
将2000个斯普拉格-道利（SD，同基因组）或刘易斯（异基因组）胰岛当量经门静脉注射到SD大鼠受体中。移植后4天和7天，将10⁸个18F-FDG标记的脾细胞注入颈静脉。脾细胞取自未致敏或致敏（门静脉内移植2000个刘易斯胰岛当量12天后）的SD大鼠。脾细胞输注后5分钟开始正电子发射断层扫描（PET）成像，并每小时进行1次，共进行4小时。
结果
脾细胞注射后1小时，FDG主要在心脏和肺部被检测到。随后它进一步分布到其他器官，从第2小时起，示踪剂浓度最高的部位位于腹部。胰岛移植后4天和7天，同基因组、异基因组和致敏异基因组肝脏的FDG摄取相似。
讨论
18F-FDG-白细胞成像未检测到胰岛排斥反应。移植组织的量只有几毫升，排斥反应时额外的相关炎症很小且难以检测。肝脏显示出相对较高的自发示踪剂摄取；相关背景妨碍了在胰岛排斥反应情况下检测示踪剂摄取的潜在增加。
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