Guo Jingfei, He Kanglai, Hellmich Richard L, Bai Shuxiong, Zhang Tiantao, Liu Yunjun, Ahmed Tofael, Wang Zhenying
State Key Laboratory for Biology of Plant Diseases and Insect Pests, MOA - CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
USDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA 50011, USA.
Sci Rep. 2016 Feb 26;6:22102. doi: 10.1038/srep22102.
Possible non-target effect of transgenic cry1Ie maize exerts on natural enemy community biodiversity in the field is unresolved. In the present study, a 2-yr comparison of transgenic cry1Ie maize (Event IE09S034, Bt maize) and its near isoline (Zong 31, non-Bt maize) on natural enemy community biodiversity were compared with whole plant inspections, pitfall traps and suction sampler. Natural enemy diversity indices (Shannon-Wiener', Simpson's and Pielou's index) and abundance suggested there were no significant differences between the two types of maize. The only exceptions were the Pielou's index for whole plant inspections in 2013 and abundance for pitfall traps in 2012, which were significantly higher in Bt maize than those of non-Bt maize. The main species of natural enemies were identical in Bt and non-Bt maize plots for each method and the three methods combined. For whole plant inspections, Bt maize had no time-dependent effect on the entire arthropod natural enemy community, and also no effect on community dissimilarities between Bt and non-Bt maize plots. These results suggested that despite the presence of a relatively minor difference in natural enemy communities between Bt and non-Bt maize, transgenic cry1Ie maize had little, if any, effect on natural enemy community biodiversity.
转基因cry1Ie玉米对田间天敌群落生物多样性可能产生的非靶标效应尚未得到解决。在本研究中，通过全株检查、陷阱诱捕器和吸虫采样器，对转基因cry1Ie玉米（事件IE09S034，Bt玉米）及其近等基因系（综31，非Bt玉米）的天敌群落生物多样性进行了为期两年的比较。天敌多样性指数（香农-威纳指数、辛普森指数和皮洛指数）和丰度表明，两种类型的玉米之间没有显著差异。唯一的例外是2013年全株检查的皮洛指数和2012年陷阱诱捕器的丰度，Bt玉米中的这些指标显著高于非Bt玉米。对于每种方法以及三种方法综合起来，Bt和非Bt玉米地块中的主要天敌种类相同。对于全株检查，Bt玉米对整个节肢动物天敌群落没有时间依赖性影响，对Bt和非Bt玉米地块之间的群落差异也没有影响。这些结果表明，尽管Bt和非Bt玉米之间的天敌群落存在相对较小的差异，但转基因cry1Ie玉米对天敌群落生物多样性几乎没有影响（如果有影响的话也很小）。
