Shakoor Sadia, Reller Megan E, LeFevre Amnesty, Hotwani Aneeta, Qureshi Shahida M, Yousuf Farheen, Islam Mohammad Shahidul, Connor Nicholas, Rafiqullah Iftekhar, Mir Fatima, Arif Shabina, Soofi Sajid, Bartlett Linda A, Saha Samir
Department of Paediatrics and Child Health, Division of Women & Child Health, The Aga Khan University, Stadium Road, P.O. Box 3500, Karachi, Pakistan.
Department of Pathology and Laboratory Medicine, The Aga Khan University, Stadium Road, P.O. Box 3500, Karachi, Pakistan.
Reprod Health. 2016 Feb 25;13:15. doi: 10.1186/s12978-016-0121-4.
The South Asian region has the second highest risk of maternal death in the world. To prevent maternal deaths due to sepsis and to decrease the maternal mortality ratio as per the World Health Organization Millenium Development Goals, a better understanding of the etiology of endometritis and related sepsis is required. We describe microbiological laboratory methods used in the maternal Postpartum Sepsis Study, which was conducted in Bangladesh and Pakistan, two populous countries in South Asia.
METHODS/DESIGN: Postpartum maternal fever in the community was evaluated by a physician and blood and urine were collected for routine analysis and culture. If endometritis was suspected, an endometrial brush sample was collected in the hospital for aerobic and anaerobic culture and molecular detection of bacterial etiologic agents (previously identified and/or plausible).
The results emanating from this study will provide microbiologic evidence of the etiology and susceptibility pattern of agents recovered from patients with postpartum fever in South Asia, data critical for the development of evidence-based algorithms for management of postpartum fever in the region.
南亚地区是世界上孕产妇死亡风险第二高的地区。为了预防因败血症导致的孕产妇死亡，并根据世界卫生组织千年发展目标降低孕产妇死亡率，需要更好地了解子宫内膜炎及相关败血症的病因。我们描述了在孟加拉国和巴基斯坦这两个南亚人口众多的国家开展的孕产妇产后败血症研究中所使用的微生物学实验室方法。
方法/设计：由医生对社区产后发热的孕产妇进行评估，并采集血液和尿液进行常规分析和培养。如果怀疑患有子宫内膜炎，则在医院采集子宫内膜刷样本进行需氧和厌氧培养以及对细菌病原体（先前已鉴定和/或可能的病原体）进行分子检测。
本研究的结果将为从南亚产后发热患者中分离出的病原体的病因及药敏模式提供微生物学证据，这些数据对于制定该地区产后发热的循证管理算法至关重要。
