Iizuka Y, Sakurai E, Hikichi N, Niwa H
Yakugaku Zasshi. 1989 Sep;109(9):680-4. doi: 10.1248/yakushi1947.109.9_680.
The activities of lysosomal maltase in the serum, bile and liver were determined in intrahepatic cholestasis rats induced by alpha-naphtylisothiocyanate (ANIT, 200 mg/kg, i.p.), and compared with changes in alkaline phosphatase (ALP) activity. Moreover, the influences of endogenous bile acids on the release of maltase activity from the liver in intrahepatic cholestasis rats were studied. The maltase activities in the serum and bile significantly increased from 4 and 8 h after the intraperitoneal administration of ANIT, respectively. Conversely, a significant decrease in liver maltase activity was observed from 4 h after the injection of ANIT. On the other hand, total bile acid concentrations in the serum and bile significantly increased immediately after the treatment of ANIT, when biliary bile acid, exogenous bile acid or Triton X-100 was added to lysosomal fraction in the liver, the maltase activity in the supernate after the reaction significantly increased in proportion to the concentration of each substance added to the liver lysosome. These results suggested that maltase might be released from liver lysosomal membrane by surface active-action of bile acid accumulated in the liver after the administration of ANIT. Moreover, the changes in ALP activities in the serum, bile and liver after the administration of ANIT were almost similar to those in maltase activity.
测定了由α-萘异硫氰酸酯（ANIT，200mg/kg，腹腔注射）诱导的肝内胆汁淤积大鼠血清、胆汁和肝脏中溶酶体麦芽糖酶的活性，并与碱性磷酸酶（ALP）活性的变化进行比较。此外，研究了内源性胆汁酸对肝内胆汁淤积大鼠肝脏麦芽糖酶活性释放的影响。腹腔注射ANIT后4小时和8小时，血清和胆汁中的麦芽糖酶活性分别显著增加。相反，注射ANIT后4小时观察到肝脏麦芽糖酶活性显著降低。另一方面，ANIT处理后血清和胆汁中的总胆汁酸浓度立即显著增加，当将胆汁酸、外源性胆汁酸或 Triton X-100 添加到肝脏溶酶体组分中时，反应后上清液中的麦芽糖酶活性与添加到肝脏溶酶体中的每种物质的浓度成比例显著增加。这些结果表明，ANIT给药后肝脏中积累的胆汁酸的表面活性作用可能使麦芽糖酶从肝溶酶体膜释放出来。此外，ANIT给药后血清、胆汁和肝脏中ALP活性的变化与麦芽糖酶活性的变化几乎相似。
