Fahmy Sohair R, Sayed Dawlat A, Soliman Amel M, Almortada Nesreen Y, Aal Wafaa E Abd-El
Zoology Department, Faculty of Science, Cairo University, Gamaa Street, Giza, Egypt.
Zoology Department, Faculty of Science, Sana'a University, El wahda street, Sana'a, Yemen.
Braz J Biol. 2020 Jan-Feb;80(1):102-111. doi: 10.1590/1519-6984.192697.
The present study was designed to evaluate the protective effects of echinochrome (Ech) on intrahepatic cholestasis in rats induced by a single (i.p.) injection of alpha-naphthylisothiocyanate (ANIT) (75 mg/kg body weight). The rats were pre-treated orally for 48hr (one dose / 24hr) with Ech (1, 5 and 10 mg/kg body weight) or ursodeoxycholic acid (UDCA) 80 mg/kg body weight drug then, injected with ANIT. ANIT markedly increased serum activities of alanine amino transaminase (ALT), aspartate amino transaminase (AST) and alkaline phosphatase (ALP), which was accompanied by a massive inflammation of epithelial cells on bile duct at 24h after ANIT injection. ANIT also increased the levels of total protein (TP), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin (IB), however decrease albumin content (ALB). In addition ANIT increased hepatic MDA and NO level and decreased GSH level and GST activity. The Ech exerted hepatoprotective and anticholestatic effects as assessed by a significant decrease in the activities of serum AST, ALT and ALP, and the levels of TP, TB, DB and IB as well as liver MDA level and NO level. In conclusion, Ech was found to possess hepatoprotective effect against intrahepatic cholestasis induced by hepatotoxin such as ANIT.
本研究旨在评估海胆紫酮（Ech）对单次腹腔注射α-萘异硫氰酸酯（ANIT，75毫克/千克体重）诱导的大鼠肝内胆汁淤积的保护作用。大鼠口服Ech（1、5和10毫克/千克体重）或熊去氧胆酸（UDCA，80毫克/千克体重）进行预处理48小时（一剂/24小时），然后注射ANIT。ANIT显著增加血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）的活性，在注射ANIT后24小时，胆管上皮细胞伴有大量炎症。ANIT还增加了总蛋白（TP）、总胆红素（TB）、直接胆红素（DB）、间接胆红素（IB）的水平，但降低了白蛋白含量（ALB）。此外，ANIT增加了肝脏丙二醛（MDA）和一氧化氮（NO）水平，降低了谷胱甘肽（GSH）水平和谷胱甘肽S-转移酶（GST）活性。通过血清AST、ALT和ALP活性以及TP、TB、DB和IB水平以及肝脏MDA水平和NO水平的显著降低评估，Ech发挥了肝脏保护和抗胆汁淤积作用。总之，发现Ech对肝毒素如ANIT诱导的肝内胆汁淤积具有肝脏保护作用。
