预防性袖状胃切除术有助于维持db/db糖尿病小鼠的β细胞功能。
Preventative Sleeve Gastrectomy Contributes to Maintaining β Cell Function in db/db Diabetic Mouse.
作者信息
Li Feng, Sheng Chunjun, Song Kexiu, Zhang Manna, Bu Le, Yang Peng, Sheng Hui, Li Hong, Qu Shen
机构信息
Department of Endocrinology and Metabolism, Shanghai Tenth People's Hospital, Tong-Ji University, 301 Middle Yan-Chang Road, ShangHai, 200072, China.
出版信息
Obes Surg. 2016 Oct;26(10):2402-10. doi: 10.1007/s11695-016-2112-5.
BACKGROUND
We used the leptin-receptor (LPR)-deficient mice model (db/db), a spontaneous model of type 2 diabetes with early β cell dysfunction to determine whether a preventative sleeve gastrectomy (SG) is an effective technique for the treatment of β cell failure.
METHODS
The animals operated at an early stage of life, prior to metabolic alterations, were used to study the molecular mechanisms of β cell function improvement after a SG.
RESULTS
β cell function was significantly increased, and islet morphology remained normal, after the SG. The expression of Glut2, Pdx1, MafA, and Nkx6.1 were significantly increased after the SG. The expression of GLP-1 in the colonic tissue, as well as GLP-1R and PKC in islets, was significantly increased after the SG.
CONCLUSIONS
β cell dysfunction can be ameliorated by a preventative SG for db/db mice. Maintaining the GLP-1 pathway and key transcript factor (TF) activation contributes to the improvement of β cell function after a preventative SG.
背景
我们使用瘦素受体（LPR）缺陷小鼠模型（db/db），这是一种具有早期β细胞功能障碍的2型糖尿病自发模型，以确定预防性袖状胃切除术（SG）是否是治疗β细胞衰竭的有效技术。
方法
在代谢改变之前的生命早期进行手术的动物用于研究SG后β细胞功能改善的分子机制。
结果
SG后β细胞功能显著增加，胰岛形态保持正常。SG后Glut2、Pdx1、MafA和Nkx6.1的表达显著增加。SG后结肠组织中GLP-1的表达以及胰岛中GLP-1R和PKC的表达显著增加。
结论
预防性SG可改善db/db小鼠的β细胞功能障碍。维持GLP-1途径和关键转录因子（TF）激活有助于预防性SG后β细胞功能的改善。
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