Louahchi S, Allam I, Raaf N, Berkani L, Boucharef A, Abdessemed A, Khaldoun N, Bahaz N, Ladjouze-Rezig A, Nebbab A, Ghaffor M, Djidjik R
Immunology Department, Beni Messous Teaching Hospital, Algiers, Algeria.
Laboratory of Cellular and Molecular Biology, Cytokine and NO Synthase Team, USTHB, Algiers, Algeria.
HLA. 2016 Mar;87(3):160-4. doi: 10.1111/tan.12757. Epub 2016 Feb 24.
Increasing evidence suggests that the rs6822844, within KIAA1109/TENR/IL2/IL21 gene cluster on 4q27, is strongly associated with rheumatoid arthritis (RA) in the Caucasian population. The aim of this study is to investigate the possible association between the SNP rs6822844 and susceptibility to RA in the Algerian Maghreb population, and to explore the association with the clinical and immunological features of RA. The polymorphism rs6822844 was genotyped in 323 RA patients and 323 healthy individuals using the TaqMan assay. A strong association of IL2/IL21 with RA susceptibility was detected in the Algerian population [odds ratio (OR) = 2.57 (95% confidence interval (CI) 1.74-3.83), P = 10(-4) ]. Our results revealed that IL2/IL21 predisposed to disease development in both autoantibody positive and negative disease. Meanwhile, the association was stronger in RA patients with anti-cyclic citrullinated peptides (ACPA) positive than those with ACPA negative [OR = 2.30 (95% CI 1.53-3.51), P = 10(-4) and OR = 1.98 (95% CI 1.01-4.22), P = 0.037, respectively]. Moreover, our findings showed a moderate association of the rs6822844 polymorphism with disease activity (P = 0.014). This study indicates for the first time that there is a strong association between IL2/IL21 rs6822844 variant and susceptibility to RA in the Algerian population, and that this association was independent from the autoantibodies status of RA patients.
越来越多的证据表明，位于4q27的KIAA1109/TENR/IL2/IL21基因簇内的rs6822844与白种人群中的类风湿性关节炎（RA）密切相关。本研究的目的是调查单核苷酸多态性（SNP）rs6822844与阿尔及利亚马格里布人群RA易感性之间的可能关联，并探讨其与RA临床和免疫学特征的关联。采用TaqMan检测法对323例RA患者和323例健康个体进行rs6822844多态性基因分型。在阿尔及利亚人群中检测到IL2/IL21与RA易感性有很强的关联[比值比（OR）=2.57（95%置信区间（CI）1.74 - 3.83），P = 10⁻⁴]。我们的结果显示，IL2/IL21在自身抗体阳性和阴性疾病中均易导致疾病发展。同时，抗环瓜氨酸肽（ACPA）阳性的RA患者中的关联比ACPA阴性患者更强[OR分别为2.30（95%CI 1.53 - 3.51），P = 10⁻⁴和OR = 1.98（95%CI 1.01 - 4.22），P = 0.037]。此外，我们的研究结果显示rs6822844多态性与疾病活动度有中度关联（P = 0.014）。本研究首次表明，IL2/IL21 rs6822844变体与阿尔及利亚人群的RA易感性之间存在很强的关联，且这种关联独立于RA患者的自身抗体状态。
