Rytel L, Calka J
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Str. Oczapowski 13, Olsztyn, Poland.
Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Str. Oczapowski 13, Olsztyn, Poland.
Neurosci Lett. 2016 Mar 23;617:218-24. doi: 10.1016/j.neulet.2016.02.029. Epub 2016 Feb 23.
Acetylsalicylic acid is a popular drug that is commonly used to treat fever and inflammation, but which can also negativity affect the mucosal layer of the stomach, although knowledge concerning its influence on gastric innervation is very scarce. Thus, the aim of the present study was to study the influence of prolonged acetylsalicylic acid supplementation on the extrinsic primary sensory neurons supplying the porcine stomach prepyloric region. Fast Blue (FB) was injected into the above-mentioned region of the stomach. Acetylsalicylic acid was then given orally to the experimental gilts from the seventh day after FB injection to the 27th day of the experiment. After euthanasia, the nodose ganglia (NG) and dorsal root ganglia (DRG) were collected. Sections of these ganglia were processed for routine double-labelling immunofluorescence technique for substance P (SP), calcitonine gene related peptide (CGRP), galanin (GAL), neuronal isoform of nitric oxide synthase (nNOS) and vasoactive intestinal polypeptide (VIP). Under physiological conditions within the nodose ganglia, the percentage of the FB-labeled neurons immunoreactive to particular substances ranged between 17.9 ± 2.7% (VIP-like immunoreactive (LI) neurons in the right NG) and 60.4 ± 1.7% (SP-LI cells within the left NG). Acetylsalicylic acid supplementation caused a considerable increase in the expression of all active substances studied within both left and right NG and the percentage of neurons positive to particular substances fluctuated from 47.2 ± 3.6% (GAL-LI neurons in the right NG) to 67.2 ± 2.0% (cells immunoreactive to SP in the left NG). All studied substances were also observed in DRG neurons supplying the prepyloric region of the stomach, but the number of immunoreactive neurons was too small to conduct a statistical analysis. The obtained results show that ASA may influence chemical coding of the sensory neurons supplying the porcine stomach, but the exact mechanisms of this action still remain unknown.
乙酰水杨酸是一种常用药物，通常用于治疗发热和炎症，但它也会对胃黏膜层产生负面影响，尽管关于其对胃神经支配影响的了解非常有限。因此，本研究的目的是研究长期补充乙酰水杨酸对供应猪胃幽门前区域的外在初级感觉神经元的影响。将快蓝（FB）注射到上述胃区域。然后从FB注射后第7天至实验第27天对实验小母猪口服乙酰水杨酸。安乐死后，收集结状神经节（NG）和背根神经节（DRG）。对这些神经节的切片进行处理，采用常规双标免疫荧光技术检测P物质（SP）、降钙素基因相关肽（CGRP）、甘丙肽（GAL）、神经元型一氧化氮合酶（nNOS）和血管活性肠肽（VIP）。在生理条件下，结状神经节内对特定物质免疫反应的FB标记神经元百分比在17.9±2.7%（右NG中VIP样免疫反应（LI）神经元）至60.4±1.7%（左NG中SP-LI细胞）之间。补充乙酰水杨酸导致左、右NG内所有研究的活性物质表达显著增加，对特定物质呈阳性的神经元百分比在47.2±3.6%（右NG中GAL-LI神经元）至67.2±2.0%（左NG中对SP免疫反应的细胞）之间波动。在供应胃幽门前区域的DRG神经元中也观察到了所有研究物质，但免疫反应神经元数量太少，无法进行统计分析。所得结果表明，阿司匹林可能影响供应猪胃的感觉神经元的化学编码，但其确切作用机制仍不清楚。
