Okano K, Yonehara Y, Yaga K, Ishida Y, Yamamoto K
Rinsho Byori. 1989 Jul;37(7):840-4.
Identification of the lineage and maturation stage of the leukemic cells is essential for the diagnosis, treatment and followup of acute leukemia. Staining of blood smear by avidin-biotin-peroxidase complex method, which requires no special equipments, enables to observe both morphology and surface antigens at the same time. Simultaneous expression of a B-lymphocyte antigen J5 and a platelet antigen GP IIb/IIIa complex on the same blasts was demonstrated by this technique in the present case.
识别白血病细胞的谱系和成熟阶段对于急性白血病的诊断、治疗及随访至关重要。抗生物素蛋白-生物素-过氧化物酶复合物法对血涂片进行染色,无需特殊设备,可同时观察形态和表面抗原。在本病例中,通过该技术证实在同一母细胞上同时表达B淋巴细胞抗原J5和血小板抗原GP IIb/IIIa复合物。