Forlenza Michael J, Latimer Jean J, Baum Andrew
Division of Behavioral Medicine and Oncology, University of Pittsburgh Cancer Institute, 3600 Forbes Ave, Suite 405, Pittsburgh, PA 15213.
University of Pittsburgh Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee Women's Research Institute.
Psychol Health. 2000;15(6):881-891. doi: 10.1080/08870440008405589. Epub 2007 Dec 19.
Research has shown that lymphocytes of high-distress patients have reduced DNA repair relative to that of low-distress patients and healthy controls. Furthermore, deficits in repair are associated with an increased risk of cancer. Using and academic stress model, we hypothesized that students would exhibit lower levels of Nucleotide Excision Repair (NER) during a stressful exam period when compared to a lower stress period. Participants were 19 healthy graduate level students. NER was measured in lymphocytes using the unscheduled DNA synthesis (UDS) assay with slide autoradiography. Contrary to prediction, mean values for NER significantly during the higher stress period relative to the lower stress period controlling for background differences in repair. Furthermore, lymphocytes had significantly increased repair of endogenous damage during the higher stress period. Stress appears to directly increase DNA repair. Additionally, stress may increase DNA repair indirectly by increasing damage to DNA.
研究表明，与低压力患者和健康对照组相比，高压力患者的淋巴细胞DNA修复能力降低。此外，修复缺陷与患癌风险增加有关。使用学术压力模型，我们假设与低压力时期相比，学生在压力大的考试期间核苷酸切除修复（NER）水平会更低。参与者为19名健康的研究生。使用非预定DNA合成（UDS）测定法和玻片放射自显影术测量淋巴细胞中的NER。与预测相反，在控制修复背景差异的情况下，相对于低压力时期，高压力时期NER的平均值显著升高。此外，在高压力时期，淋巴细胞对内源性损伤的修复显著增加。压力似乎直接增加DNA修复。此外，压力可能通过增加对DNA的损伤间接增加DNA修复。
