Zhang Fuming, Xie Jin, Linhardt Robert J
Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York 12180, USA.
Jacobs J Biotechnol Bioeng. 2014;1(1):002. Epub 2014 Sep 4.
Glycosaminoglycans (GAGs) are linear, highly negatively charged polysaccharides. They are ubiquitous molecules exhibiting a wide range of biological functions with numerous applications in pharmaceutical, cosmetic, and nutraceutical industrials. The commercial fish-processing industry generates large quantities of solid waste, which can represent a potential resource for GAG production. In this study, we used a three-step recovery and purification scheme for isolation of GAGs from the heads of red salmon (). The GAGs recovery yield was 6 to 7 mg from 1 gram of salmon head powder. The recovered GAGs were structurally analyzed with polyacrylamide gel electrophoresis and by disaccharide composition analysis with reversed-phase ion-pair high-performance liquid chromatography. The analyses showed the major composition of the GAGs in red salmon head were chondroitin sulfate C and E.
糖胺聚糖（GAGs）是线性的、高度带负电荷的多糖。它们是普遍存在的分子，具有广泛的生物学功能，在制药、化妆品和营养保健品工业中有众多应用。商业鱼类加工业产生大量固体废物，这可能是生产GAGs的潜在资源。在本研究中，我们采用三步回收和纯化方案从红鲑鱼头中分离GAGs。从1克鲑鱼头粉中回收的GAGs产量为6至7毫克。通过聚丙烯酰胺凝胶电泳和反相离子对高效液相色谱法进行二糖组成分析，对回收的GAGs进行结构分析。分析表明，红鲑鱼头中GAGs的主要成分是硫酸软骨素C和E。
