Semiz Gürkan, Çelik Gurbet, Gönen Erhan, Semiz Aslı
a Faculty of Arts & Sciences, Department of Biology , Pamukkale University , Denizli , Turkey.
b Medical Laboratory Techniques, Vocational School of Health Services , Pamukkale University , Denizli , Turkey.
Nat Prod Res. 2016 Oct;30(19):2225-9. doi: 10.1080/14786419.2016.1149703. Epub 2016 Feb 26.
The present study was designed to examine the chemical composition of the essential oil, in vitro antioxidant activity and total phenolic and flavonoid content of extracts from plant parts (leaf, flower and stem) of Teucrium alyssifolium. The principle components of the essential oil were trans-β-caryophyllene (16.87%), ar-curcumene (11.43%) and bisabolene (11.06%), representing 39.36% of the oil. The total phenolic contents ranged between 13.99 and 41.54 mg of GAE/g of extract. The concentrations of flavonoids varied from 16.82 to 49.52 mg of Ru/g of extract. Antioxidant activity was determined in vitro using DPPH reagent and expressed as concentration of each extract required to inhibit radical by 50% (IC50) values that ranged from 13.52 to 132.55 μg/ml. Our results have indicated that water extract of T. alyssifolium (part leaf) with a total content of polyphenols (41.54 mg of GAE/g) and an IC50 of 13.52 μg/ml is more antioxidant.
本研究旨在检测香雪球叶石蚕(Teucrium alyssifolium)植物各部位(叶、花和茎)提取物的精油化学成分、体外抗氧化活性以及总酚和黄酮含量。精油的主要成分是反式-β-石竹烯(16.87%)、芳姜黄烯(11.43%)和没药烯(11.06%),占精油的39.36%。总酚含量在13.99至41.54毫克没食子酸当量/克提取物之间。黄酮类化合物的浓度在16.82至49.52毫克芦丁/克提取物之间变化。使用DPPH试剂在体外测定抗氧化活性,并表示为将自由基抑制50%(IC50)所需的每种提取物浓度,其范围为13.52至132.55微克/毫升。我们的结果表明,香雪球叶石蚕叶水提取物的多酚总含量为(41.54毫克没食子酸当量/克),IC50为13.52微克/毫升,具有更强的抗氧化性。