Stauffer G V, Stauffer L T, Plamann M D
Department of Microbiology, University of Iowa, Iowa City 52242.
Mol Gen Genet. 1989 Dec;220(1):154-6. doi: 10.1007/BF00260870.
A glycine cleavage enzyme system, inducible by glycine, has been demonstrated in Salmonella typhimurium. The induced enzyme levels, however, are only about 20% of the induced levels found in Escherichia coli. Starting with a serine auxotroph, mutants were isolated that grow with a serine supplement, but not with a glycine supplement. Three independently isolated mutants have reduced or nondetectable glycine cleavage enzyme levels. The new mutations, designated gcv, were mapped between the serA and lys genes at 62.5 min on the S. typhimurium chromosome.
在鼠伤寒沙门氏菌中已证实存在一种可被甘氨酸诱导的甘氨酸裂解酶系统。然而，诱导后的酶水平仅约为大肠杆菌中诱导水平的20%。从一个丝氨酸营养缺陷型出发，分离出了一些突变体，这些突变体在添加丝氨酸时能够生长，但在添加甘氨酸时不能生长。三个独立分离的突变体的甘氨酸裂解酶水平降低或检测不到。这些新的突变被命名为gcv，它们被定位在鼠伤寒沙门氏菌染色体上62.5分钟处的serA和lys基因之间。
