Rodrigues Helena, Carvalho Maria Isabel, Pires Isabel, Prada Justina, Queiroga Felisbina L
CECAV, University of Porto , Porto , Portugal.
Department of Veterinary Sciences, University of Porto , Porto , Portugal.
Acta Vet Hung. 2016 Mar;64(1):78-89. doi: 10.1556/004.2016.009.
Fifty canine mammary gland tumours (CMGT) (18 benign and 32 malignant) were studied by immunohistochemical detection of active caspase-3 and Ki-67 antigens in order to determine their association with several clinicopathological parameters. The percentage of caspase-3 positive cells was significantly higher in benign tumours as compared to their malignant counterparts (P ≤ 0.001). In the group of malignant tumours there was no significant association between active caspase-3 and the clinicopathological variables considered. The percentage of Ki- 67 positive cells was significantly higher in malignant tumours compared to the benign ones (P ≤ 0.001). In the group of malignant tumours, Ki-67 expression showed a statistically significant association with tumour size (P = 0.025), histological type (P = 0.010), mitotic grade (P ≤ 0.001), nuclear grade (P = 0.025), differentiation grade (P = 0.004), histological grade of malignancy (P = 0.002), and presence of metastases in regional lymph nodes (P = 0.025). Furthermore, this study revealed a negative correlation between the percentages of active caspase-3 and Ki-67 (r = -0.39; P = 0.04). Thus, our results suggest a loss of balance between cell death and cell division in CMGT.
Apoptosis, caspase-3, Ki.
对50例犬乳腺肿瘤（CMGT）（18例良性和32例恶性）进行了研究，通过免疫组织化学检测活性半胱天冬酶-3和Ki-67抗原，以确定它们与几个临床病理参数的关联。与恶性肿瘤相比，良性肿瘤中半胱天冬酶-3阳性细胞的百分比显著更高（P≤0.001）。在恶性肿瘤组中，活性半胱天冬酶-3与所考虑的临床病理变量之间没有显著关联。与良性肿瘤相比，恶性肿瘤中Ki-67阳性细胞的百分比显著更高（P≤0.001）。在恶性肿瘤组中，Ki-67表达与肿瘤大小（P = 0.025）、组织学类型（P = 0.010）、有丝分裂分级（P≤0.001）、核分级（P = 0.025）、分化分级（P = 0.004）、恶性组织学分级（P = 0.002）以及区域淋巴结转移的存在（P = 0.025）显示出统计学上的显著关联。此外，本研究揭示了活性半胱天冬酶-3和Ki-67百分比之间的负相关（r = -0.39；P = 0.04）。因此，我们的结果表明犬乳腺肿瘤中细胞死亡和细胞分裂之间失去了平衡。
细胞凋亡，半胱天冬酶-3，Ki
