在活跃生长的前列腺组织中，缺氧诱导因子-1α（HIF-1α）的表达升高与良性前列腺增生的临床特征相关。

Elevated expression of HIF-lα in actively growing prostate tissues is associated with clinical features of benign prostatic hyperplasia.

作者信息

Wu Fei, Ding Sentai, Li Xin, Wang Hui, Liu Shuai, Wu Haihu, Bi Dongbin, Ding Kejia, Lu Jiaju

机构信息

Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.

Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.

出版信息

Oncotarget. 2016 Mar 15;7(11):12053-62. doi: 10.18632/oncotarget.7641.

BACKGROUND

Benign prostatic hyperplasia (BPH) is one of the most common diseases in middle-age or older men. Increasing evidence has shown that BPH is associated with hypoxia microenvironment.

METHODS

We retrospectively collected patient data and tissue samples from fetal prostates(FP), normal prostates(NP), intra-acinar of BPH, peri-acinar of BPH, prostate cancers and sarcomas of prostate. The expression of HIF-1α, as well as VEGF was visualized by immunohistochemistry and statistically analyzed with clinical parameters.

RESULTS

Expression of HIF-lα was observed in intra-acinar of BPH (69.5%), prostate cancer (85.7%) and all FPs, while NP and peri-acinar of BPH tissues were all stained negative. HIF-lα levels in FPs and the malignant tumors were higher than BPH tissues(p < 0.05), and the expression of HIF-lα in intra-acinar of BPH was higher than NP and peri-acinar of BPH (p < 0.05). The expression of HIF-lα was correlated with the weight of intra-acinar of prostate (p < 0.05). And patients with prostate weight larger that 72.45g were prone to have HIF-lα moderate-positive expression, according to the ROC curve (AUC = 0.734, 95%CI = 0.630-0.838). Moreover, the risk of acute urine retention (AUR) for HIF-lα moderate-positive patients increased significantly (OR=5.517, 95%CI = 2.434-12.504).

CONCLUSIONS

HIF-lα expression is increased in highly proliferative prostate tissues and correlated with the weight of intra-acinar prostate. Moreover, HIF-lα is also an independent risk factor for AUR occurrence in BPH patients.

背景

良性前列腺增生（BPH）是中老年男性最常见的疾病之一。越来越多的证据表明，BPH与缺氧微环境有关。

方法

我们回顾性收集了来自胎儿前列腺（FP）、正常前列腺（NP）、BPH腺泡内、BPH腺泡周围、前列腺癌和前列腺肉瘤的患者数据及组织样本。通过免疫组织化学观察缺氧诱导因子-1α（HIF-1α）以及血管内皮生长因子（VEGF）的表达，并与临床参数进行统计学分析。

结果

在BPH腺泡内（69.5%）、前列腺癌（85.7%）以及所有胎儿前列腺中均观察到HIF-1α的表达，而NP以及BPH腺泡周围组织均呈阴性染色。胎儿前列腺和恶性肿瘤中的HIF-1α水平高于BPH组织（p<0.05），且BPH腺泡内的HIF-1α表达高于NP和BPH腺泡周围（p<0.05）。HIF-1α的表达与前列腺腺泡内重量相关（p<0.05）。根据ROC曲线（AUC=0.734，95%CI=0.630-0.838），前列腺重量大于72.45g的患者易于出现HIF-1α中度阳性表达。此外，HIF-1α中度阳性患者发生急性尿潴留（AUR）的风险显著增加（OR=5.517，95%CI=2.434-12.504）。