van Genderen E, Clabbers M T B, Das P P, Stewart A, Nederlof I, Barentsen K C, Portillo Q, Pannu N S, Nicolopoulos S, Gruene T, Abrahams J P
Biophysical Structural Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
Nanomegas SPRL, Boulevard Edmond Machtens 79, B 1080, Brussels, Belgium.
Acta Crystallogr A Found Adv. 2016 Mar;72(Pt 2):236-42. doi: 10.1107/S2053273315022500. Epub 2016 Feb 5.
Until recently, structure determination by transmission electron microscopy of beam-sensitive three-dimensional nanocrystals required electron diffraction tomography data collection at liquid-nitrogen temperature, in order to reduce radiation damage. Here it is shown that the novel Timepix detector combines a high dynamic range with a very high signal-to-noise ratio and single-electron sensitivity, enabling ab initio phasing of beam-sensitive organic compounds. Low-dose electron diffraction data (∼ 0.013 e(-) Å(-2) s(-1)) were collected at room temperature with the rotation method. It was ascertained that the data were of sufficient quality for structure solution using direct methods using software developed for X-ray crystallography (XDS, SHELX) and for electron crystallography (ADT3D/PETS, SIR2014).
直到最近，通过透射电子显微镜对束敏感的三维纳米晶体进行结构测定时，为了减少辐射损伤，需要在液氮温度下收集电子衍射断层扫描数据。本文表明，新型Timepix探测器结合了高动态范围、非常高的信噪比和单电子灵敏度，能够对束敏感的有机化合物进行从头相位分析。使用旋转法在室温下收集了低剂量电子衍射数据（约0.013 e⁻ Å⁻² s⁻¹）。经确定，这些数据的质量足以使用为X射线晶体学（XDS、SHELX）和电子晶体学（ADT3D/PETS、SIR2014）开发的软件，通过直接法来解析结构。
